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Method for preparing chitin and chitosan from dendrolimus punctatus larvae

A technology for chitin and chitosan, which is applied in the field of preparing chitin and chitosan, can solve the problems of harming pine forests, narrow market application fields, harming pine forests, etc., and achieves the effects of easy collection, widening the source of raw materials, and reducing the amount of harm.

Inactive Publication Date: 2009-10-28
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Chinese published patent CN1186811A uses Yunnan pine caterpillar pupae as raw material to prepare chitin and chitosan. The patent uses: sodium hydroxide concentration of 40-50%, deacetylation reaction at 90-110°C for 10-20 hours , the above process can only prepare chitin with a deacetylation degree of less than 70%, and its product market application field is narrow
In addition, because the pine caterpillar harms the pine forest mainly in the larval stage, the pupa is collected as raw material after the larval stage, and the pine caterpillar has caused serious harm to the pine forest at this time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 1 kg of the old larvae of Dendrolimus punctatus, soak them in 15 times the volume of 1% NaCl solution for 2 hours, filter, and layer the larvae with each layer thickness not exceeding 2 cm. Place them in a common oven at 75°C for 6 hours. Then put the treated Dendrolimus punctatus larvae into a 20-mesh gauze bag, squeeze it in water for 0.5h until the skins and contents are separated, wash with tap water and filter to obtain 242g of skins. Insect skins were soaked in 15 times volume, 6% NaOH solution at 75℃ for 10h, after washing with tap water, then 15 times volume, 4% hydrochloric acid solution was soaked at 50℃ for 7h, after washing, 10 times volume, 9 times % Hydrogen peroxide was decolorized at 50°C for 4 hours, washed with distilled water, and dried to obtain 206 g of white flake solid chitin. The moisture content of the product is 8.89%, the ash content is 0.93%, the nitrogen content is 6.84%, and the yield is 20.6%.

[0024] The chitin product was further immer...

Embodiment 2

[0026] Take 1 kg of old larvae of Dendrolimus punctatus, and the method of detoxification and skin separation is the same as in Example 1. Insect skins were soaked in 15 times the volume of 8% NaOH solution at 78°C for 14 hours. After washing with tap water, they were soaked in 15 times the volume of 3% hydrochloric acid solution at 40°C for 8 hours. After washing with tap water, use 10 times the volume, 10% hydrogen peroxide was decolorized at 40°C for 4 hours, washed with distilled water, and dried to obtain 203 g of white flake solid chitin. The moisture content of the product is 8.89%, the ash content is 0.89%, the nitrogen content is 6.78%, and the yield is 20.3%.

[0027] The chitin product is further immersed in 15 times the volume, 40% NaOH solution for 10 hours to deacetylate, washed with tap water to neutral, then treated with 5% dilute hydrochloric acid solution for 6 hours, washed with distilled water to neutral, and dried to obtain 183g white flakes Solid chitosan. Th...

Embodiment 3

[0029] Take 1 kg of old larvae of Dendrolimus punctatus, and the method of detoxification and skin separation is the same as in Example 1. Insect skins were soaked in 15 times the volume of 10% NaOH solution at 75℃ for 12h. After washing with tap water, they were soaked in 15 times the volume of 6% hydrochloric acid solution at 45℃ for 6 hours. After washing with tap water, use 10 times the volume, 10% hydrogen peroxide was decolorized at 40°C for 4 hours, washed with distilled water, and dried to obtain 200 g of white flake solid chitin. The moisture content of the product is 8.32%, the ash content is 0.81%, the nitrogen content is 6.59%, and the yield is 20.0%.

[0030] The chitin product is further immersed in 15 times the volume, 50% NaOH solution for 6h to deacetylate, washed with tap water to neutral, then treated with 5% dilute hydrochloric acid solution for 6h, washed with distilled water to neutral, dried to get 179g white flakes Solid chitosan. The moisture content of th...

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Abstract

The invention discloses a method for preparing chitin and chitosan from dendrolimus punctatus larvae which are major pests. The dendrolimus punctatus larvae undergoes the steps of detoxification treatment, peel separation, organic matter removing treatment, mineral removing treatment and decoloring treatment to obtain the chitin and then undergoes deacetylation treatment to obtain the chitosan. The chitin and chitosan preparing method expands the source for chitin and chitosan preparation; and as the dendrolimus punctatus larvae is widely available, the preparation cost is low. In addition, by directly developing the larvae, the method makes the harmful pests profitable, remarkably reduces the loss in the forest industry and is suitable for wide application.

Description

Technical field [0001] The invention belongs to the field of biological resource processing, and specifically relates to a method for preparing chitin and chitosan from larvae of Dendrolimus punctatus. Background technique [0002] Chitin, also known as chitin, chitin, etc. Chitosan is the product of chitin removed part of the acetyl group. Chitin and chitosan are natural non-toxic polymer polysaccharide substances, which have been widely used in many fields such as medicine, chemical industry, biology, agriculture, textile, printing and dyeing, papermaking, and environmental protection. The domestic and foreign markets often appear in short supply. At present, the annual output of chitosan in my country is only about 200 tons, and the demand is 450 tons, which is far from meeting market demand. [0003] At this stage, the main raw materials used to prepare chitosan in the industry are shrimp shells and crab shells discarded from aquatic processing plants, such as Chinese publish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 刘高强魏美才蒋晓宇周国英章怀云
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY