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Method for extracting polygonatum odoratum polysaccharide

An extraction method and polysaccharide technology are applied in the extraction field of Polysaccharide Polysaccharide, which can solve the problems of excessive amount of extraction solvent, waste, and long extraction time, and achieve the effects of shortening the crushing time, reducing the amount of polysaccharide, and shortening the extraction time.

Inactive Publication Date: 2009-11-18
宽甸玉竹农副产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the reported Polygonatum odoratum extraction methods are decoction method, reflux extraction method, percolation method, etc., which have the characteristics of long extraction time and large amount of extraction solvent, and there is a certain waste in the large-scale production process, but at present I have not seen related reports about Guan Yuzhu in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Guan Yuzhu crude drug powder is passed through a 60-mesh sieve, and 100 g of powder under the sieve is taken, and 1500 g of water is added, and at 20° C., KQ-100DV type numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), ultrasonic frequency 30 kHz, Extraction time 0.5h. Extract 3 times, filter, combine the three extracts, concentrate under reduced pressure to a relative density of 1.36 at a vacuum pressure of 0.08MPa and a temperature of 25°C, put it at room temperature, add 3 times of absolute ethanol, shake well, and let it stand for 12h , filtered, separated the precipitate, washed 3 times with 0.5 times of acetone, discarded the washing solution, and freeze-dried and pulverized the precipitate at -50°C according to the conventional method to obtain 19.96 g of white powder. The polysaccharide yield was determined to be 8.79%.

Embodiment 2

[0020] Example 2: Polygonatum officinalis crude drug powder was passed through a 60-mesh sieve, 100 g of the under-sieved powder was taken, 3000 g of water was added, the extraction temperature was 50° C., the ultrasonic frequency was 80 kHz, and the extraction time was 2 h. Extract twice, filter, combine the extracts, concentrate under reduced pressure to a relative density of 1.18, put it at room temperature, add 4 times of absolute ethanol, shake well, let it stand for 24 hours, filter, separate the precipitate, and wash with 1.5 times of acetone 2 times, the washing liquid was discarded, and the precipitate was freeze-dried and pulverized at -50°C according to the conventional method to obtain 20.32 g of white powder. The polysaccharide yield was determined to be 8.97%.

Embodiment 3

[0021] Example 3: Polygonatum officinalis crude drug powder was passed through a 60-mesh sieve, 100 g of the under-sieved powder was taken, 4000 g of water was added, the extraction temperature was 80° C., the ultrasonic frequency was 100 kHz, and the extraction time was 2 hours. Extract once, filter, concentrate the extract under reduced pressure to a relative density of 1.29, put it at room temperature, add 3 times of absolute ethanol, shake well, let it stand for 36 hours, filter, separate the precipitate, and wash with 3 times of acetone for 1 The second time, the washing solution was discarded, and the precipitate was freeze-dried and pulverized at -50°C according to a conventional method to obtain 22.15 g of white powder. The yield of polysaccharide was determined to be 9.36%.

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Abstract

The invention provides a method for extracting polygonatum odoratum polysaccharide. The method comprises that: polygonatum odoratum crude drug powder is extracted by ultrasonic; water is used as an extracting agent; the extracting temperature is between 20 and 80 DEG C; the ultrasonic frequency is between 30 and 100kHz; the powder is filtered after extracting; the extract is decompressed and concentrated; absolute ethanol is added into the extract to separate precipitate; and the precipitate is washed by acetone, frozen, dried and crushed. Ultrasonic extraction is adopted, so the extract has small dosage, the quantity of polysaccharide dissolved in the extract is reduced, the yield of the polysaccharide is improved, the extracting temperature is reduced by 10 to 20 DEG C compared with the prior method, and low temperature extraction is favorable for protecting effective ingredients. Meanwhile, the extracting time is shortened by over 1 hour. Compared with conventional methods, such as the decocting method, the immersion method, the percolation method and the like, the ultrasonic extraction method has the advantages of time conservation, energy conservation, high extracting efficiency and the like.

Description

technical field [0001] The invention relates to a method for extracting plant polysaccharides, in particular to the extraction of polygonatum polysaccharides. Background technique [0002] In recent years, with the research progress of immune substances, biofilms and various biologically active substances, it has been shown that the functions of carbohydrates in living organisms are not limited to providing energy and participating in structures, but also have a variety of biological functions. It participates in various activities of cells, especially the research on polysaccharides has become a hot spot of concern. Scholars from various countries have been actively studying and extracting polysaccharides with certain physiological activities from various biological materials, so that polysaccharides Research has an unprecedented and rapid development trend. With the in-depth understanding of the activity of polysaccharides and people's admiration for various natural medic...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04
Inventor 赵鑫朱丹宁伟张波吴杰
Owner 宽甸玉竹农副产品有限公司
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