Method for controlling the growth and reproduction of pasteurella and use thereof

A pasteurella and mycelium technology, applied in the field of regulating the development and reproduction of bacteria, can solve the problems of immature in vitro culture technology and difficulty in large-scale production, etc.

Inactive Publication Date: 2009-11-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the bacteria has not been widely used in production. The main reason is that the in vitro culture technology is still immature, and it mainly relies on living hosts for reproduction, so it is difficult to produce on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the regulation of temperature on the development of Pasteurella puncture

[0018] 1. The regulation of temperature on the development of Pasteurella puncture

[0019] The regulation of temperature on the development of Pasteurella puncture is carried out according to the following steps:

[0020] 1. Test nematodes and their isolation

[0021] The tested nematode was the root-knot nematode Meloidogyne spp. (China Forestry Microbial Culture Collection and Management Center). Take the tomato plants that have been infected with root knot nematodes, wash the roots of the tomato with water, place them in 0.5% sodium hypochlorite solution for 30 sec, quickly scrub, sieve, rinse, and collect the root knot nematode eggs on a 3-layer filter paper. In a nematode separator, incubate at room temperature for 3-4 days to obtain the second instar larvae of root-knot nematodes.

[0022] 2. Pasteurella piercing root knot nematode

[0023] The root knot nematodes cultiva...

Embodiment 2

[0051] Embodiment 2, the regulation of temperature on the development of Pasteurella puncture

[0052] 1. The regulation of temperature on the development of Pasteurella puncture

[0053] The regulation of temperature on the development of Pasteurella puncture is carried out according to the following steps:

[0054] 1. Test nematodes and their isolation

[0055] The tested nematode was the root-knot nematode Meloidogyne spp. (China Forestry Microbial Culture Collection and Management Center). Take the tomato plants that have been infected with root knot nematodes, wash the roots of the tomato with water, place them in 0.5% sodium hypochlorite solution for 30 sec, quickly scrub, sieve, rinse, and collect the root knot nematode eggs on a 3-layer filter paper. In a nematode separator, incubate at room temperature for 3-4 days to obtain the second instar larvae of root-knot nematodes.

[0056] 2. Pasteurella piercing root knot nematode

[0057] The root knot nematode cultured...

Embodiment 3

[0085] Embodiment 3. The regulation of temperature on the development of Pasteurella nishizawae

[0086] 1. Regulation of temperature on the development of Pasteuria nishizawae

[0087] The regulation of temperature on Pasteuria nishizawae is carried out according to the following steps:

[0088] 1. Test nematodes and their isolation

[0089] The tested nematodes were cyst nematodes (Heterodera spp., ) (China Forestry Microbial Culture Collection and Management Center). Take the soybean plants that have been infected with cyst nematodes, pick fresh cysts of cyst nematodes on soybean roots under a stereoscopic microscope, select fresh, plump and mature cysts and oocysts and disinfect them in 0.5% NaOCl solution for 3 minutes, sterile Rinse with water 3 times, then incubate in sterile water at 25°C, and collect newly hatched cyst nematodes in time every day.

[0090] 2. Pasteuria nishizawae Adherent cyst nematode

[0091] With the cyst nematode cultured in the above step (1)...

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Abstract

The invention discloses a method for controlling the growth and reproduction of pasteurella and use thereof. In the method, pasteurella is cultured at a temperature of equal to or greater than 8 DEG C and less than 20 DEG C. The invention controls the growth of pasteurella by adjusting the temperature so as to overcome the problem of difficult and complicated obtaining of mycelium through in vitro culture, thereby ensuring the smooth development of in vitro culture in the aspect of material so as to save large amount of workload for preparation of inoculating material and effectively solve the problem of large space needed by the inoculating material for reproducing living bodies. By using the method in the invention, the pasteurella can be preserved for about 30 days, and after the pasteurella regulated through this method is transferred to the normal condition, the activity of the mycelium does not change; and when the effective accumulated temperature reaches 400 DEG C, the mycelium can grow to form spores without affecting the yield and quality of the spores. The invention is also applicable to controlling the growth and preserving other pasteurella of other strains, and also applicable to controlling the growth and preserving the pasteurella in artificial culture medium.

Description

technical field [0001] The invention relates to a method for regulating the development and reproduction of bacteria, in particular to a method for regulating the development and reproduction of Pasteurella. Background technique [0002] Pasteuria spp. is a class of parasitic plant parasitic nematodes, bacteria capable of producing hyphae and endospores, and belongs to the low GC content Gram-positive branch of eubacteria. Pasteurella is an obligate parasite of the nematode that completes its growth and reproduction process inside the nematode and is released back into the soil in the form of spores. Endospores are extremely resistant to stress in the soil. When the host nematodes pass through, the endospores attach to the nematode body wall, germinate as the nematodes develop, and invade into the nematode prosthesis cavity to absorb their nutrients. , destroy its tissue cells, and eventually lead to sluggish action of nematode larvae and weakened infectivity, or reduce the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/01
Inventor 简恒卜祥霞陈忠孝何强刘倩
Owner CHINA AGRI UNIV
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