Identification method for quickly detecting purity and truth of rice seeds

A rice seed and identification method technology, which is applied in the field of rapid detection of rice seed purity and authenticity identification, can solve the problems of seed authenticity that cannot be effectively solved, complicated, and not stable enough for analysis

Inactive Publication Date: 2009-11-25
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the problem of seed authenticity cannot be effectively solved
[0006] RAPD technology has broad application prospects in germplasm identification. It requires a small amount of sample DNA, high reaction sensitivity, no tissue and developmental stag...

Method used

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  • Identification method for quickly detecting purity and truth of rice seeds
  • Identification method for quickly detecting purity and truth of rice seeds
  • Identification method for quickly detecting purity and truth of rice seeds

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Embodiment Construction

[0134] An identification method for quickly detecting the purity and authenticity of rice seeds by using molecular markers, the specific steps are as follows:

[0135] 1. Extraction of rice genomic DNA:

[0136] DNA was extracted using Murray and Thompson's CTAB method (Nucleic Acids Res. 1980, 8: 4321-4325), briefly as follows:

[0137](1) Selection of IRRI011, IRRI012, IRRI013, IRRI014, IRRI016, IRRI022, IRRI025, IRRI029, IRRI030, IRRI031, IRRI046, IRRI050, IRRI053, IRRI057, IRRI060, IRRI066, IRRI078, IRRI081, IRRI09786, IRRI and IRRI111 total of 22 farm species (see the table below), take 0.1g of rice tender leaves, cut into pieces of about 0.3cm×0.5cm and put them into a 1.5ml centrifuge tube, then add an ordinary steel ball with a diameter of 3mm, Grind the leaves into a slurry on a cell disrupter (TissueLyser II, RETSCH, Germany), add 500 μl of CTAB extract, and bathe in 65° C. water bath for 25-30 minutes.

[0138] Numbering

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Abstract

The invention discloses an identification method for quickly detecting purity and truth of rice seeds. The method comprises the following steps of firstly extracting the DNA of a sample; and then, performing PCR amplification detection on the extracted DNA by an SCAR marker in the patent; and finally, comparing and analyzing the amplified DNA segment. The marker in the invention is used for identifying the purity and truth of rice seeds, not only has the basic characteristics of environmental stability, identifiability of inter-variety variation, smallest intra-variety variation and reliability of experimental result, but also has the advantages of quickness, convenience and low cost on application, and is applied to construction of specific fingerprint picture of plant variety, seed purity quick analysis of large samples, detection and identification of fake and inferior variety, molecular marker-assisted breeding and gene clone.

Description

technical field [0001] The present invention relates to the technical field of molecular marker detection, and more specifically to a method for rapid qualitative and rapid analysis of the purity of large samples of rice seeds, identification of counterfeit and shoddy varieties, and construction of specific fingerprints of multiple varieties. The method is also applicable to molecular Marker-assisted selection breeding, and at the same time, SCAR markers can be used as probes to screen genomic libraries and be applied to map-based cloning to construct contigs. Background technique [0002] Rice is the largest food crop in my country, especially hybrid rice, which accounts for about 60% of the total rice planting area, has a large planting area and high profit from seed sales, and occupies a very important position in my country's food production. In the process of rice variety breeding and hybrid rice seed production, the fertility instability of the sterile line or the rest...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李姝婧谢红卫李绍清朱英国
Owner WUHAN UNIV
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