Pharmaceutical composition containing renin inhibitor, diuretic agent and folic acid and application thereof
A technology of renin inhibitor and composition, which is applied to the pharmaceutical composition containing renin inhibitor, diuretic and folic acid and its application field, can solve the problem of unknown efficacy, side effects and low bioavailability of aliskiren/hydrochlorothiazide, etc. To achieve the effect of reducing the risk of cardiovascular and cerebrovascular events, taking medicines easily, and reducing medical costs
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Embodiment 1
[0042] Example 1: Protective effect of Aliskiren + indapamide + folic acid on reducing blood pressure and target organ damage in spontaneously hypertensive rats
[0043] SHR rats, weighing 150-180g, were divided into 6 groups on average, 20 rats in each group, including model group, aliskiren (15mg / kg) group, indapamide (0.25mg / kg) group, aliskiren +Indapamide (15+0.25mg / kg) group, Aliskiren+Folic acid (15+0.04mg / kg) group, Aliskiren+Indapamide+Folic acid (15+0.25+0.04mg / kg) group, and another 20 normal rats were taken as the normal control group. Give the same volume of 0.5% CMC solution, weigh once a week, adjust the dosage according to the body weight, and administer the medicine continuously for 20 weeks.
[0044] Detection Indicator:
[0045] (1) Measure the systolic blood pressure of rat tails at different times before administration and after administration, and compare the differences in blood pressure among the groups.
Embodiment 2
[0068] Example 2: Protective effect of aliskiren + indapamide + folic acid on target organ damage in stroke-susceptible spontaneously hypertensive rats
[0069] A total of 80 male SHR-SP rats aged about 8 weeks were divided into model group, aliskiren+indapamide+folic acid (30+0.25+0.08mg / kg) group, aliskiren+folic acid group according to blood pressure value. (30+0.08mg / kg) group, Aliskiren (30mg / kg) group. Breeding conditions: room temperature 22±2°C; relative humidity 50%; solid feed and litter were provided by the Experimental Animal Breeding Farm of the Chinese Academy of Medical Sciences; drinking water was pure water. Another WKY control group was set up, with 20 rats in each group. Oral administration, once a day, weighed once a week, adjust the dose according to body weight, for 8 consecutive weeks.
[0070] Observation indicators:
[0071] (1) The diet, survival and behavior of the animals were observed daily, and the occurrence of cerebral apoplexy in each group ...
Embodiment 3
[0092] Example 3: Antihypertensive and target organ protection effects of teragiren + spironolactone + folic acid on hypertensive rats with two kidneys and one clip
[0093] Rats successfully modeled with two kidneys and one clip were divided into 6 groups on average, 20 in each group. + spironolactone (30+2.5mg / kg) group, teragiren + folic acid (30+0.04mg / kg) group, teragiren (30mg / kg) group, spironolactone (2.5mg / kg) group, another 20 normal rats were used as normal control group. Give the same volume of 0.5% CMC solution, weigh once a week, adjust the dosage according to the body weight, and administer the medicine continuously for 20 weeks.
[0094] Detection Indicator:
[0095] (1) Measure the systolic blood pressure of rat tails at different times before administration and after administration, and compare the differences in blood pressure among the groups.
[0096] (2) Collect urine, use turbidimetry to measure urine protein, radioimmunoassay to measure 24h urine α1 ...
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