Pharmaceutical composition containing uretic, statin and folic acid as well as applications thereof
A composition and drug technology, applied in the direction of drug combination, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of undiscovered diuretics, statin lipid-lowering drugs and folic acid combination, and achieve synergistic protection of endothelium Effect of cell function, reduction effect, effect of strengthening stiffness
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Embodiment 1
[0044] Example 1: Target organ protection effect of hydrochlorothiazide + atorvastatin + folic acid on hypertensive rats with dyslipidemia
[0045] 1. Preparation of compound model animals
[0046] For SD rats, the experiment was started after 5 days of adaptive feeding. Two kidneys and one clip operation were performed to prepare 2K1C hypertensive model animals. After feeding for 6 weeks, measure the blood pressure of the rats (measuring three times to get the average value). If the blood pressure > 140mmHg, the model is considered successful, and the model group begins to gavage fat emulsion (cholesterol 10g, propylene glycol 20ml, bile salt 2g, Tween-8020ml) every day. , propylthiouracil 1g, peanut oil 20ml, sucrose 20g, after dissolving, add distilled water to 200ml) 10ml / kg and free drinking water (containing 1% methionine), after 4 weeks of intragastric administration of fat emulsion (retest once after 2 weeks of infusion of fat emulsion) Blood pressure) The rats were ...
Embodiment 2
[0081] Example 2: Target organ protection effect of indapamide + simvastatin + folic acid on hypertensive rats with dyslipidemia
[0082] 1. Preparation of compound model animals
[0083] For SD rats, the experiment was started after 5 days of adaptive feeding. Two kidneys and one clip operation were performed to prepare 2K1C hypertensive model animals. After feeding for 6 weeks, measure the blood pressure of the rats (measuring three times to get the average value). If the blood pressure > 140mmHg, the model is considered successful, and the model group begins to gavage fat emulsion (cholesterol 10g, propylene glycol 20ml, bile salt 2g, Tween-8020ml) every day. , propylthiouracil 1g, peanut oil 20ml, sucrose 20g, after dissolving, add distilled water to 200ml) 10ml / kg and free drinking water (containing 1% methionine), after 4 weeks of intragastric administration of fat emulsion (retest once after 2 weeks of infusion of fat emulsion) Blood pressure) The rats were fasted for...
Embodiment 3
[0120] Example 3: Target organ protection effect of spironolactone + pitavastatin + folic acid on hypertensive rats with dyslipidemia
[0121] 1. Preparation of compound model animals
[0122]For SD rats, the experiment was started after 5 days of adaptive feeding. Two kidneys and one clip operation were performed to prepare 2K1C hypertensive model animals. After feeding for 6 weeks, the blood pressure of the rats was measured (measured three times to get the average value). If the blood pressure > 140mmHg, the model was considered successful, and the model group began to gavage fat emulsion (cholesterol 10g, propylene glycol 20ml, bile salt 2g, Tween-80 20ml, 1g of propylthiouracil, 20ml of peanut oil, 20g of sucrose, after dissolving, add distilled water to 200ml) 10ml / kg and free drinking water (containing 1% methionine), after 4 weeks of intragastric administration of fat emulsion (retest after 2 weeks of infusion of fat emulsion) One blood pressure) The rats were fasted...
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