Synthesis method of ractopamine artificial antigen

Abstract

A synthesis method of ractopamine artificial antigen is characterized by linking ractopamine with activated para aminobenzoic acid to prepare ractopamine active intermediate containing carboxyl, adopting ethyl[3-(dimethylamino)propyl]carbodiimide as crosslinker to couple the ractopamine active intermediate with the amino of carrier protein, and dialyzing and freeze drying the product to obtain ractopamine artificial antigen. In the synthesis method of the invention, ractopamine can be coupled with carrier protein successfully so that ractopamine artificial antigen-antibody absolute immunogen can be prepared which can prompt an immune response in animals.

Description

technical field [0001] The invention relates to a beta-stimulant drug, in particular to a method for synthesizing an artificial antigen of ractopamine. Background technique [0002] Ractopamine is a β-agonist drug, which has a similar structure to other β-agonist drugs such as clenbuterol, carbuterol, and salbutamol. As we all know, β-stimulants can affect the flow and redistribution of nutrients in animals, can effectively promote the growth of muscle tissue, reduce carcass fat content, increase lean meat rate and increase lean meat production, so they have been widely used in Europe and the United States . However, because β-stimulants have a chemical structure and mechanism of action similar to adrenaline, they can bind to β-receptors on the cell surface and cause symptoms such as palpitations, headaches, dizziness, nausea, vomiting, tremors, nervousness, vasodilation, heart rate, etc. Acceleration and other physiological effects, and this type of compound has oral activ...

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Problems solved by technology

However, because β-stimulants have a chemical structure and mechanism of action similar to adrenaline, they can bind to β-receptors on the cell surface and cause symptoms such as palpitations, headaches, dizziness, nausea, vomiting, tremors, nervousness, vasodilation, heart rate, etc. Acceleration and other physiological effects, and this type of compound has oral activity, if used in excess, it will cause certain toxic and side effects on the liver, kidney and other internal organs of the body. At present, European and American countries prohibit its application in animal husbandry. For scientific research, the Ministry of Agriculture of my country also strictly prohibits the use of such drugs as additives

However, driven by economic interests, illegal use still exists in animal husbandry production, resulting in residues of such drugs in animals, and the export of livestock and poultry products has repeatedly encountered green barriers, which has damaged the international reputation of my country's export products and affected the health of animal husbandry. development, causing great economic losses to the entire industry

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