Indoor detection method for resistance of peanuts to infection of Aspergillus flavus

A detection method and technology for Aspergillus flavus, applied in the agricultural field, can solve the problems of low efficiency, long infection time and high cost, and achieve the effects of improving detection efficiency, reducing pollution risks and reducing usage.

Inactive Publication Date: 2009-12-23
SHANDONG PEANUT RES INST
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Problems solved by technology

[0007] (Purpose of the invention) The purpose of this method is to identify the resistance to Aspergillus flavus infection of different peanut variety resources, solve the problems of high cost, long infection time, and low efficiency in the current As...

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  • Indoor detection method for resistance of peanuts to infection of Aspergillus flavus

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[0022] After the tested peanut seed resources are harvested, use 7-9 days of sunlight and air flow to evaporate the water in the pods. In case of cloudy and rainy weather, use a drying machine to accelerate the water distribution in the range of 35-40°C to a water content of 8-10°C. %, stored at room temperature;

[0023] The Aspergillus Flavus strain (Aspergillus Flavus) is prepared with sterile water at a concentration of 0.05~1×10 8 Individual / mL spore suspension;

[0024] The dried peanut seeds were treated with 0.8% HgCl 2 The solution is sterilized for 6-8 minutes, and rinsed with sterile water for 3-4 times, and the water content of the seeds is restored to 15-20%. For peanut seeds, cut the seed coat horizontally and uniformly drill holes in the vertical cotyledon section at the largest grain diameter to create wounds for Aspergillus flavus infection;

[0025] Autoclave the petri dish and filter paper at 121°C, put the filter paper into the petri dish and add spore s...

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Abstract

An indoor detection method for resistance of peanuts to infection of Aspergillus flavus includes the following steps of: drying the harvested seeds of peanuts to reach the water content of 8-10 percent, blending the bacterial strain of Aspergillus flavus into spore suspension with the concentration of 0.05-1*10 pieces/mL; sterilizing the dried peanut seeds with a regular method and bleaching the seeds with sterile water to recover the water content of the seeds to 15-20 percent; creating a cut ready for the infection of Aspergillus flavus on the peanut seeds by adopting a method of transverse girdling of testa and uniformly drilling a place which has the maximum diameter of kernel and is perpendicular to the section of seed leaf; putting filter paper in a culture dish, adding the spore suspension to moisten the filter paper, soaking the treated peanut seeds in the spore suspension for 3-5min, taking out the seeds, then using sterile filter paper to suck the surfaces of the seeds dry, putting the seeds on the filter paper of the culture dish, subsequently shielding the culture dish with a dish cover, and putting the culture dish in an incubator to cultivate the seeds for 5-6days under the culture conditions including humidity being 80-90 percent and temperature being 28-30 DEG C; and evaluating the resistance according to the result that the kernel surface and hole of peanuts are infected with the Aspergillus flavus.

Description

field of invention [0001] The invention relates to the technical field of agriculture, in particular to an indoor rapid and efficient detection method for peanut resistance to Aspergillus flavus infection. Background technique [0002] Peanut is one of the five largest oil crops in the world, and it is also an important oil crop and economic crop in my country. Aflatoxin is a secondary metabolite produced by Aspergillus flavus infecting peanuts. It can induce experimental liver cancer in humans and various animals. It is the strongest chemical carcinogen discovered so far. Aflatoxin pollution caused by Aspergillus flavus infecting peanuts is the main factor affecting the production, processing and trade of peanuts in my country. What's more serious is that 95% of the peanuts used for domestic consumption are much more seriously contaminated with aflatoxin than the peanuts exported, posing a serious threat to the health of domestic consumers, which has become a limiting fact...

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Application Information

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IPC IPC(8): C12Q1/18C12R1/67
Inventor 单世华孔青李春娟陈高张廷婷闫彩霞
Owner SHANDONG PEANUT RES INST
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