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Method for preparing plasma polypeptide

A plasma and blood cell technology, applied in the field of bioengineering, can solve the problems of incomplete blood meal raw materials, and achieve the effects of easy automatic control, high extraction efficiency and simple operation

Inactive Publication Date: 2009-12-30
张吉越
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method ferments and degrades the raw material of blood meal incompletely

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: A kind of preparation method of plasma polypeptide, comprises the following several steps: (a) slow down the chicken blood of frozen agglomeration, separate into plasma and blood cell with centrifuge; (b) use high-power Ultrasonic crusher to break the cell wall; (c) mix the plasma and the broken blood cells and add water to dissolve to form a plasma blood cell mixed solution; (d) heat the plasma blood cell mixed solution to 85°C and keep it warm for 30 minutes to sterilize; (e) Lower the temperature of the sterilized mixed solution of plasma and blood cells to 38°C, add trypsin, and incubate for 3 hours; (f) heat the mixed solution of plasma and blood cells after enzymatic digestion with trypsin to 48°C, add alkaline protease, Heat preservation enzymolysis for 5 hours; (g) heat the mixed solution of plasma and blood cells after alkaline protease enzymolysis to 60°C, add papain, and heat preservation enzymolysis for 8 hours; (h) heat plasma after three kinds...

Embodiment 2

[0020] Embodiment 2: A method for preparing plasma polypeptides, comprising the following steps: (a) slowing down the frozen clotted pig blood, and separating it into plasma and blood cells with a centrifuge; (b) using a high-power Ultrasonic crusher to break the cell wall; (c) mix the plasma and the broken blood cells and add water to dissolve to form a plasma blood cell mixed solution; (d) heat the plasma blood cell mixed solution to 90°C and keep it warm for 20 minutes to sterilize; (e) Lower the temperature of the sterilized mixed solution of plasma and blood cells to 39°C, add trypsin, and incubate for 3.5 hours for enzymolysis; (f) heat the mixed solution of plasma and blood cells after enzymatic digestion with trypsin to 50°C, add alkaline protease, Heat preservation enzymolysis for 4 hours; (g) heat the mixed solution of plasma and blood cells after alkaline protease enzymolysis to 62°C, add papain, and heat preservation enzymolysis for 7 hours; (h) heat plasma after th...

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PUM

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Abstract

The invention relates to a method for preparing a plasma polypeptide, which comprises the following steps: subjecting animal blood to cell wall broking, mixing, dissolution, sterilization, enzymolysis by trypsinase, basic protease and papain and enzyme inactivation; and filtering the resulting product by a molecular film filter and drying the resulting product to obtain plasma polypeptide powder. The preparation method is simple in operation, implementable at normal temperature and pressure, energy-saving, environmentally-friendly, low in cost and high in plasma polypeptide extraction rate, realizes the high polypeptide content of the product, and greatly improves the digestion and conversion rate of animal proteins.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for preparing plasma polypeptides from animal blood. technical background [0002] Animal blood is a precious protein resource with high nutritional value. It has a wide range of uses and is suitable for feed, food, health care products, etc. The market demand is large, the prospect is broad, and the product value is high. At present, there are many researches on blood meal in foreign countries. The traditional utilization methods of blood meal include steaming method, thermal denaturation treatment method and spray drying method, etc., and more modern methods include acid hydrolysis method, fermentation method and enzymatic method. Acid hydrolysis is to use acid to hydrolyze animal blood protein, which improves the digestibility of blood meal, but destroys an essential amino acid in blood meal—tryptophan; fermentation method is to insert specific microbial strain...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 张吉越
Owner 张吉越