Forage liquid phytase preparation

A liquid phytase and phytase proenzyme technology, which is applied in the field of liquid phytase preparations for feed, can solve the problems of poor enzyme activity storage stability, reduced activity, and easy to be affected by physical and chemical factors, etc. Effects on storage stability

Active Publication Date: 2010-01-06
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the original phytase enzyme solution is diluted with water to form a liquid dosage form, there is a problem of poor storage stability of the enzyme activity
This is because the enzyme molecules are in a stretched state in the liquid state, the active center is exposed, and it is easily affected by physical and chemical factors, which reduces the activity.

Method used

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  • Forage liquid phytase preparation
  • Forage liquid phytase preparation
  • Forage liquid phytase preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 600-30kg of polyethylene glycol, 50kg of trehalose, and 0.5kg of sodium benzoate, while stirring, slowly add to 600kg of distilled water, control the temperature below 40°C, and after all the solids are dissolved, add 300kg of plant with an enzyme activity of 23270u / g Stir the phytase solution evenly, then adjust the pH to 4.5 with 20kg of 0.5M HCl to obtain 1000.5kg of phytase solution with an enzyme activity of 6981u / g. Such as image 3 , Figure 4 Shown are the stability analysis of the liquid phytase preparation prepared according to the formula of this example stored at room temperature for 6 months and at 40° C. for 6 weeks. After analysis and calculation, the enzyme activity retention rate of this product is 90% when stored at room temperature for 6 months, and the enzyme activity retention rate is 83% when stored at a constant temperature of 40°C for 6 weeks.

Embodiment 2

[0030] Weigh 600-40kg of polyethylene glycol, 75kg of trehalose, and 1kg of sodium benzoate, while stirring, slowly add to 600kg of distilled water, control the temperature below 40°C, after all the solids are dissolved, add 260kg of plant with an enzyme activity of 25880u / g Stir the phytase solution evenly, then adjust the pH to 5.0 with 4kg of 0.5M NaOH and 20kg of 0.5M HCl to obtain 1000kg of phytase solution with an enzyme activity of 6729u / g. Such as Figure 5 , Figure 6 Shown are the stability analysis of the liquid phytase preparation prepared according to the formula of this example stored at room temperature for 6 months and at 40° C. for 6 weeks. After analysis and calculation, the enzyme activity retention rate of this product is 80% when stored at room temperature for 6 months, and the enzyme activity retention rate is 82.8% when stored at a constant temperature of 40°C for 6 weeks.

Embodiment 3

[0032] Weigh 600-50kg of polyethylene glycol, 100kg of trehalose, and 1.5kg of sodium benzoate, while stirring, slowly add to 600kg of distilled water, control the temperature to less than 40°C, and after all the solids are dissolved, add 250kg of the enzyme activity of 27836u / g Stir the phytase solution evenly, then adjust the pH to 5.5 with 0.5kg of 0.5M NaOH to obtain 1002kg of phytase solution with an enzyme activity of 6959u / g. Such as Figure 7 , Figure 8 Shown are the stability analysis of the liquid phytase preparation prepared according to the formula of this example stored at room temperature for 6 months and at 40° C. for 6 weeks. According to analysis and calculation, the enzyme activity retention rate of this product stored at room temperature for 6 months is 84%, and the enzyme activity retention rate of 6 weeks at 40°C is 82.7%.

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Abstract

The invention discloses a forage liquid phytase preparation with high enzyme activity retention rate. The forage liquid phytase preparation is prepared by adding polyethylene glycol 6000, trehalose, sodium benzoate and pure water to original phytase enzyme liquid; and adjusting a pH value to 4.5-5.5, wherein hydroxide radicals in the polyethylene glycol 6000 are bonded with carboxyls, aminos, and the like on the surfaces of an enzyme protein molecule and an activity center thereof by covalent bonds, hydrogen bonds and Van der Waals force so that the activity center of the enzyme protein molecule and the whole enzyme protein molecule are protected; the trehalose (stabilizing agent) improves the permeation pressure of enzyme solution to reduce the stretching degree of the enzyme protein molecule, and the enzyme protein molecule is relatively in a dewatering state to shrink, thereby improving the stability; and the sodium benzoate (preservative) takes the effect of improving the biological stability of liquid enzymes and the pH range is optimally selected from 4.5 to 5.5 . The invention is used for the field of fodder additives.

Description

technical field [0001] The invention relates to a feed liquid enzyme preparation, in particular to a feed liquid phytase preparation. Background technique [0002] The content of phosphorus in plant feed varies, but most of it exists in the form of phytic acid and phytate. Phytate phosphorus accounts for more than 70% of the total phosphorus in plant feed raw materials. These phosphorus are difficult to be digested and utilized by monogastric animals. Unused phosphorus is excreted with feces, causing phosphorus pollution to the environment. In addition, phytic acid also reduces the utilization rate of Zn, Mn, Fe, Ca and other mineral elements and protein by animals through chelation. Phytic acid and phytate are therefore a natural anti-nutritional factor. Adding phytase to the feed and replacing some or all of the inorganic phosphorus with phytase can reduce the total phosphorus content in the feed, reduce feed costs, and improve economic benefits. At the same time, it can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/16A23K20/189
Inventor 眭培林肖渲雄
Owner GUANGDONG VTR BIO TECH
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