Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration
A phosphate radical and inorganic phosphorus technology, applied in the field of determination of inorganic phosphorus concentration, inorganic phosphorus diagnosis/measurement kit, can solve the problem of high results
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0045] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:
[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0047] Stabilizer 500mmol / L
[0048] Coenzyme 3mmol / L
[0049] Pyruvate carboxylase 10000U / L
[0050] Glycerol kinase 12000U / L
[0051] Glycerol-3-phosphate dehydrogenase 12000U / L
[0052] Adenosine diphosphate 5mmol / L
[0053] Oxaloacetate 9mmol / L
[0054] Glycerin 15mmol / L
[0055] Magnesium chloride 2mmol / L
[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0057]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured inorganic phosphorus (phosphate) sample to t...
Embodiment 2
[0060] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is double reagent, comprises:
[0061] Reagent 1
[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0063] Stabilizer 50mmol / L
[0064] Coenzyme 3mmol / L
[0065] Adenosine diphosphate 5mmol / L
[0066] Oxaloacetate 9mmol / L
[0067] Glycerin 15mmol / L
[0068] Magnesium chloride 2mmol / L
[0069] Reagent 2
[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0071] Stabilizer 50mmol / L
[0072] Pyruvate carboxylase 10000U / L
[0073] Glycerol kinase 12000U / L
[0074] Glycerol-3-phosphate dehydrogenase 12000U / L
[0075] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0076] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test seco...
Embodiment 3
[0079] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is three reagents, comprises:
[0080] Reagent 1
[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0082] Stabilizer 50mmol / L
[0083] Coenzyme 3mmol / L
[0084] Adenosine diphosphate 5mmol / L
[0085] Oxaloacetate 9mmol / L
[0086] Glycerin 15mmol / L
[0087] Magnesium chloride 2mmol / L
[0088] Reagent 2
[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0090] Stabilizer 500mmol / L
[0091] Glycerol kinase 12000U / L
[0092] Glycerol-3-phosphate dehydrogenase 12000U / L
[0093] Reagent 3
[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0095] Stabilizer 500mmol / L
[0096] Pyruvate carboxylase 10000U / L
[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0098] When measuring the con...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com