Culture medium composition suitable for germ-free germination of orchid seeds and method thereof

Abstract

The invention mainly relates to a culture medium composition suitable for germ-free germination of orchid seeds and a method thereof. Various components of a basic culture medium and a seed germination culture medium are as follows: 500mg / L of calcium nitrate, 200mg / L of potassium chloride, 20-30g / L of sucrose or white sugar and 5-7g / L of agar, wherein the basic culture medium is selected from self-improved MS, i.e. on the basis of the original MS culture medium, 440mg / L of calcium chloride in the MS basic culture medium is replaced into 500mg / L of calcium nitrate and 200mg / L of potassium chloride; and the pH value is 5.0-5.6. The seed germination culture medium comprises the basic culture medium, 0.5-1.0mg / L of 6-BA, 0.5-1.0mg / L of NAA, 1.0g / l of active carbon and 50-100m / L of coconut milk. The invention has the advantages that: (1) the orchid group-cultured optimized culture medium has strong pertinence, good adaptability and high seed germination rate; (2) the method greatly shortens the breeding period of orchid so as to rapidly obtain new genetic improvement; and (3) the method is convenient for operation and reduces the pollution to the minimum extent.

Description

A kind of aseptic germination medium composition suitable for Cymbidium seeds and method thereof technical field The invention relates to the technical field of aseptic sowing of plant seeds, in particular to a suitable aseptic germination medium composition for Cymbidium seeds and a method thereof. Optimal medium formula for aseptic germination of Cymbidium seeds and a method for promoting germination. Background technique Cymbidium faberi, also known as Xiahui, Jiujielan, etc., is a large species of Chinese orchids. Because of its strong fragrance, beautiful flower shape and rare famous products, it is regarded as a high-end potted flower in Southeast Asia. Cymbidium grows naturally in hillside forests, and most of the cultivated varieties are selected from the natural variation of wild species. Due to the long-term indiscriminate digging by humans and the continuous deterioration of the living environment of orchids, coupled with their extremely slow reproduction spee...

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Problems solved by technology

However, in the research on the aseptic germination of Cymbidium seeds in the above article, it is only roughly mentioned that the selected basic medium is improved MS, and the specific formula for improving MS is not disclosed; The method is more cumbersome to operate and can easily cause pollution

In all the documents of existing reports, there are still problems such as low germination rate of Cymbidium seeds (less than about 10%), long germination cycle (half a year to more than a year), complicated sowing procedures (seeing my report), making it impossible to realize Large-scale factory production

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