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Culture medium of euglena gracilis and open type high-density culture method

A technology of euglena and culture medium, which is applied in the field of culture medium for culturing euglena, can solve the problems of difficult industrial production, slow growth of autotrophic culture, and microbial contamination such as bacteria, so as to avoid microbial pollution and facilitate accumulation , The effect of increasing the ability to absorb CO2

Inactive Publication Date: 2010-01-27
北京芳能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autotrophic culture grows slowly, which is not conducive to the accumulation of high biomass
At present, Euglena artificial medium is mostly used for heterotrophic culture, which contains organic nutrients such as yeast extract, beef extract and peptone. Microbial pollution, difficult to achieve industrial production

Method used

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  • Culture medium of euglena gracilis and open type high-density culture method
  • Culture medium of euglena gracilis and open type high-density culture method
  • Culture medium of euglena gracilis and open type high-density culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. The formula of EM medium is as follows:

[0043] Ammonium chloride 0.8g / L Urea 60mg / L

[0044] Dipotassium hydrogen phosphate 1.0g / L Magnesium sulfate 0.2g / L

[0045] Calcium chloride 0.02g / L Ferric sulfate 3mg / L

[0046] Disodium EDTA 0.48mg / L

[0047] Trace elements 1ml / L, wherein the preparation method of trace elements is: take 1.8g of manganese chloride, 1.5g of cobalt sulfate, 0.4g of zinc sulfate, 0.2g of sodium molybdate, and 0.02g of copper sulfate, add distilled water to make up to 1L.

[0048] Vitamin B 1 0.1mg / L vitamin B 12 0.5μg / L

[0049] The above substances were dissolved, the volume was adjusted to 1 L with distilled water, the pH was adjusted to 3.5 with hydrochloric acid, and autoclaved at 121 °C for 20 min.

[0050] Add 100ml of EM medium to a 250ml shake flask and inoculate at a concentration of 5 x 10 5 Cells / ml were cultured under the conditions of temperature of 25°C, light:dark=12:12, light intensity of 5000 LUX, and shaking spe...

Embodiment 2

[0061] The EM medium formulation is as follows:

[0062] Ammonium chloride 0.2g / L

[0063] Urea was 0, 20, 40, 60, 80, 100 and 120 mg / L

[0064] Dipotassium hydrogen phosphate 1.2g / L Magnesium sulfate 0.2g / L

[0065] Calcium chloride 0.02g / L Ferric sulfate 3mg / L

[0066] Disodium EDTA 0.48mg / L

[0067] Trace elements 0.5ml / L, wherein the preparation method of trace elements is: take

[0068] 1.8g of manganese chloride, 1.6g of cobalt sulfate, 0.5g of zinc sulfate, 0.2g of sodium molybdate, and 0.02g of copper sulfate, add distilled water to make up to 1L.

[0069] Vitamin B 1 0.25mg / L vitamin B 12 20μg / L

[0070] The above substances were dissolved, the volume was adjusted to 1 L with distilled water, the pH was adjusted to 3.5 with hydrochloric acid, and autoclaved at 121 °C for 20 min. Add 100 ml of EM medium to a 250 ml shake flask with CO (NH 2 ) 2 The initial concentrations were 0, 20, 40, 60, 80, 100 and 120 mg / L, respectively, and the inoculation conce...

Embodiment 3

[0072] The EM medium was formulated as follows:

[0073] Ammonium chloride 1.6g / L Urea 60mg / L

[0074] Dipotassium hydrogen phosphate 1.5g / L Magnesium sulfate 0.4g / L

[0075] Calcium chloride 0.02g / L Ferric sulfate 10mg / L

[0076] Disodium EDTA 5mg / L

[0077] Trace element 1.5ml / L, the formula of trace element is: manganese chloride 1.8g, cobalt sulfate 1.5g, zinc sulfate 0.4g, sodium molybdate 0.2g, copper sulfate 0.02g

[0078] Vitamin B 1 3mg / L Vitamin B 12 25μg / L;

[0079] In a 400L biophotoreactor system, add EM medium, the filling volume is 90%, and the density of inoculated algae is 2 × 10 5 18% CO at a flow rate of 0.6-1L / min 2 gas, so that the pH of the system is controlled at 3 to 5, the temperature is controlled at 25 °C, the illumination is controlled at 18000-30000 LUX, and the illumination time is controlled at 12 hours / day. The results show that Euglena slenderis grows well in this photobioreactor system. On the 3rd day of culture, it entered th...

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Abstract

The invention provides a culture medium of euglena gracilis, which is added with different inorganic nutrient substances, in particular to nutrient components such as a mixture of ammonium chloride and urea, and the like. In an open culture process, the culture medium not only overcomes the defects that euglena gracilis is easy to pollute by microorganisms and bacteria so as to pollute the environment, and the like in the prior art, but also can ensure that the euglena gracilis quickly utilizes the inorganic nutrient components to accelerate cell division under the condition of not adding an organic carbon source. The culture medium of the invention shortens the grown cycle of the euglena gracilis by 2-5 days compared with a conventional culture medium and has a biomass accumulation being 1-1.5 times higher than the conventional culture medium. The culture medium removes the organic carbon source of the conventional culture medium and supplements the carbon source in the mode of CO2, thus the culture medium not only can achieve the purpose of avoiding microorganism pollution, but also can increase the capacity of absorbing the CO2 of the euglena gracilis. In the process of the mass production and culture of the euglena gracilis, the culture medium can adsorb a large amount of industrial CO2 waste gas, thereby having important meaning to energy saving, emission reduction and environmental protection.

Description

technical field [0001] The invention relates to a medium for cultivating Euglena slenderness and a method for cultivating Euglena slenderness in an open type and high density. Background technique [0002] Euglena gracilis belongs to the Euglena phylum, and is a freshwater unicellular eukaryote with bare cells without walls, spindle-shaped, 35-66 μm long, 8-18 μm wide, with 8-28 sheets or circles Disc-shaped chromosomal body, 1 flagella, about 0.15-1 times the body length, with obvious eye spots. The amino acid composition of Euglena slender protein is relatively balanced, among which 10 amino acids necessary for aquatic animals account for about 50% of its total amino acid content, and are rich in higher unsaturated fatty acids and basic fatty acids, which are high-quality bait for sea and freshwater organisms . Secondly, Euglena slenderness can also be used to produce high value-added products such as vitamin C, vitamin E and beta-carotene. In addition, Euglena slendern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 单东杰
Owner 北京芳能科技有限公司
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