Escherichia coli strain for recombined engineering

A technology of Escherichia coli and recombinant engineering, applied in the field of genetic engineering, can solve the problem of keeping the temperature uniform and difficult to control, and achieve the effect of rapid growth and high efficiency
CN101633901AInactive Publication Date: 2010-01-27NANJING NORMAL UNIVERSITY
0 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Publication Date
2010-01-27
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to escherichia coli CGMCC NO.3192 for recombined engineering and a variant thereof. The strain is obtained by integrating gene segments subjected to the recombined engineering, i.e. a regulatory gene araC of the Arabinose ara operon from the escherichia coli, a promoter pBAD of the Arabinose ara operon from the escherichia coli, recombined enzyme genes red alpha, red alpha beta and gam from a gamma bacteriophage, a gene recA and gentamicin resistance gene (Gm) from the escherichia coli, into an endA gene area of a gene group of the escherichia coli DH10B, wherein red alpha, red alpha beta, gam and recA are driven by pBAD induced by arabinose. The recombined enzyme can catalyze homologous recombination among DNA short segments to finish the recombined engineering. The length of the DNA segment is about 50 base pairs. The invention has convenient operation of carrying out the recombined engineering by the strain and high recombined efficiency, thereby becoming the universal strain for the recombined engineering.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to an Escherichia coli used for recombinant engineering. Background technique

[0002] Recombination engineering refers to a relatively novel gene cloning method for gene cloning or DNA transformation in Escherichia coli by homologous recombination between short DNA fragments catalyzed by recombinase. Recombination engineering has great advantages when conventional gene cloning methods are difficult or inefficient. For example, when the DNA fragment to be cloned is too large, it is difficult to find a suitable enzyme cutting site, it is difficult to perform gel recovery, the yield of PCR amplification is low, and it is easy to introduce mismatched bases; Gene mutations may also occur. Recombination engineering avoids these steps and has gradually become a routine cloning method.

[0003] The recombinases in recombination engineering are mainly derived from three genes of lambda...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More