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Extraction purification process of teasel root saponin

A technology of interrupted saponin and process, applied in the field of extraction and purification of interrupted saponin VI, can solve problems such as high cost, seriousness, environmental pollution, etc., and achieve the effects of low cost, avoiding hydrolysis, and avoiding environmental pollution

Inactive Publication Date: 2010-02-10
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of total saponins of Dipsacus is mainly carried out by macroporous resin purification process, which has high cost and serious environmental pollution.
However, there is no mature process for the industrialized extraction of the monomer components of saponins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh 2 kg of Dipsacus chuanxiong crude powder, infiltrate the crude powder of Dipsacus sichuan with 1000ml of 60% ethanol for 3 hours, soak in 60% ethanol for 12 hours after installing the percolation cylinder, percolate 12 times the volume, combine the filtrate, and recover the filtrate Recover under reduced pressure until there is no alcohol smell, add water to adjust the volume to a solution containing 0.5g Dipsacus extract per milliliter, centrifuge at high speed or filter to remove water-insoluble impurities to obtain a clear liquid; use 0.1% NaOH solution to adjust the pH value is about 10, extract the clear liquid 3 times with water-saturated n-butanol, combine the extracted n-butanol layers, back-extract the n-butanol layer once with n-butanol-saturated water, and recover the n-butanol layer to dryness. The residue is the total saponin extract of Dipsacus.

[0014] Under the condition of 80°C water bath, heat and dissolve the total saponin extract of Dipsacus s...

Embodiment 2

[0017] Weigh 2 kg of Dipsacus chuanxiong crude powder, soak it in 6000 ml of 60% ethanol, and warm it at 50 °C for 3 times, 1 hour each time, filter the above leachate, combine the filtrate, recover the filtrate under reduced pressure until it has no alcohol smell, add water to adjust The volume of the percolation liquid is a solution containing 0.5g Dipsacus herb extract per milliliter, high-speed centrifugation or filtration to remove water-insoluble impurities to obtain a clear liquid; extract 3 times with n-butanol saturated with water, and use n-butanol for the n-butanol layer Saturated water was back extracted once, and the n-butanol layers were combined and recovered to dryness to obtain the total saponin extract of Dipsacus cuspidatum.

[0018] Dipsacus total saponin extract is dissolved with water saturated with an appropriate amount of chloroform at room temperature, the solution is put into a separatory funnel, an appropriate amount of chloroform is added to the solu...

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Abstract

The invention discloses an extraction purification process of teasel root saponin. The technical scheme includes that: teasel medicinal material coarse powder is subject to rough extraction process toobtain teasel saponins; the teasel saponins is added with water to be dissolved, temperature or solvent polarity is reduced until the teasel saponin VI is fully sedimented, the sediment is obtained by filtering or centrifugation, and the operations are repeated for 1-3 times to obtain teasel saponin VI monomer. The invention has the beneficial effects that: the process is lower in cost compared with that macroporous resin is adopted, thus environmental pollution is avoided; water is taken as mother solution for precipitation purification, thus the influence of other glycosides in the saponinson the purity of teasel saponin VI is effectively eliminated; and an amount of organic solvent is added into precipitation mother solution, thus educt quantity can be increased while hydrolysis of saponin is avoided.

Description

technical field [0001] The patent of the present invention relates to an extraction and purification process, more specifically to an extraction and purification process of Dipsacus saponin VI. technical background [0002] The dried root of Dipsacus asperoides C.Y.Cheng et T.M.Ai is a commonly used traditional Chinese medicine and is listed as the top grade in "Shen Nong's Materia Medica". Ancient medical scientists believe that Dipsacus is bitter, sweet, pungent, slightly warm in nature, and has the functions of nourishing the liver and kidney, promoting blood circulation, and continuing injury. Clinically, it is mostly used for symptoms such as golden sores, tumbling, lumbago and soft feet, and fetal movement. Modern research has proved that Dipsacus contains various components such as alkaloids and saponins. Pharmacological experiments have reported that the total glycoside extract of Dipsacus has the activity of promoting bone healing. Among them, triterpene saponins...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07J63/00C07H1/08A61K36/185A61P19/08
Inventor 罗维早覃瑶阳勇李钰婷王海军
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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