Plate-type settling centrifuge for perfusion

Abstract

The invention discloses a plate-type settling centrifuge for perfusion, which is used for recycling cultured cells suspended in a bioreactor. A cover of the centrifuge is provided with a material inlet / recycling pipe, a liquid casting pipe, a culture medium conveying pipe and a spraying hole, all of which extend into a rotary drum and are respectively connected with a bioreactor, a supernate storage tank and a culture medium storage tank through a T-shaped valve set. A transmission principal shaft is connected with a rotary drum cavity through four in-series sealing connections comprising framework seal, mechanical sealing, water sealing and mechanical sealing. The rotary drum cavity and the connecting pipes can be sterilized by steam. The cultured material in the bioreactor is sent into the rotary drum through the material inlet / recycling pipe, the centrifugated cells settle on the rotary drum wall, supernate is absorbed out through the liquid casting pipe, the culture medium is sprayed on the settled cells on the rotary drum wall rotating slowly through the culture medium conveying pipe and the spraying hole, and the suspended cells are conveyed back into the bioreactor through the recycling / material inlet pipe.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a plate-type sedimentation centrifuge for perfusion used to recover suspension culture cells in a bioreactor Background technique [0002] Animal cell culture is generally divided into two modes: batch culture and continuous perfusion culture. Compared with batch culture, perfusion culture can supplement the balanced nutrition required for cell growth, reduce the metabolites produced by cells that inhibit cell proliferation and target protein expression, increase cell density, increase the yield of target protein in a single cell, and prolong cell culture. cycle, greatly increasing the volumetric productivity of the bioreactor. In the perfusion culture process, cell retention is a very important link. [0003] Existing cell retention equipment mainly includes tank internal (outside) rotary filter, acoustic field cell retention device, inclined cell gravity settler, etc. The a...

AI Technical Summary

Problems solved by technology

The ordinary drum centrifuges reported by Liu Changxing (ZL94227190.4), K. Stroken et al. (ZL99807460.8) and Sun Fuyi (ZL200820012627.9) are open-ended and cannot achieve aseptic separation, nor can they be separated from Docking of bioreactors for cell perfusion culture

Although the centrifuge cup in an ordinary rotor centrifuge can be aseptically sealed, its capacity is limited, and it is not suitable for the centrifugation of large-volume cultures, nor can it realize the automatic resuspension and recovery of precipitated cells

[0005] The present invention designs a new type of centrifuge, utilizes the advantage of the centrifuge to effectively realize solid-liquid separation, overcomes the shortcomings of the centrifuge's open operation and limited capacity, and designs a device for cleaning and resuspending cells to be used in biological reactions continuous perfusion culture

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