Health food containing chlorella and herbal extracts
A technology of chlorella extract, health food, applied in the field of new health food of malt and honey, which can solve problems such as irritation
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Embodiment 1
[0037] Embodiment 1: the preparation of composition of the present invention
[0038] 1. Preparation of the aqueous composition of the present invention
[0039] Chlorella extract;
[0040] Dissolve the dry chlorella powder in ethyl acetate and extract at 45°C for 10 hours.
[0041] The solution was centrifuged to obtain the supernatant.
[0042] Ethanol was added to the supernatant to precipitate the active substance.
[0043] The precipitate was filtered, washed with absolute ethanol, and the extract was dried in vacuo.
[0044] herbal extracts;
[0045] Ginseng, Ganoderma lucidum, Valerian root, Poria cocos, Dried ginger, Bupleurum and Licorice are mixed and crushed to a size of about 0.001-10mm 3 .
[0046] The pulverized mixture was added to distilled water or ethanol, and extracted at 80°C for 5 hours.
[0047] The extracted solution was centrifuged to obtain a supernatant.
[0048] malt;
[0049] After cooking the rice bran, Aspergillus Oryzae was inoculated. ...
Embodiment 2
[0072] Embodiment 2: Determination of antioxidant activity
[0073] The elimination of DPPH free radicals will cause the color change of DPPH, so the antioxidant activity is determined as follows:
[0074] 5 mg of DPPH was dissolved in 100 ml of 60% methanol to prepare a DPPH solution.
[0075] Sample solution: a mixed solution of DPPH solution (750ul) and UC (200ul).
[0076] Blank solution: a mixed solution of 60% methanol (750ul) and UC solution (200ul).
[0077] Control solution: a mixed solution of DPPH solution (750ul) and 60% methanol (200ul).
[0078] The absorbance of each solution was measured at 515 nm, respectively. Antioxidant activity (free radical scavenging activity) was calculated with the following formula:
[0079] Antioxidant activity (%)=(C-S) / (C-B)×100
[0080] C: Absorbance of the control solution
[0081] B: Absorbance of blank solution
[0082] C: Absorbance of sample solution
[0083] The above steps are respectively applied to the compositi...
Embodiment 3
[0086] Embodiment 3: Determination of hematopoietic ability
[0087] Radiation destroys bone marrow cells and has adverse effects on the development and proliferation of normal immune cells, thus reducing hematopoietic and immune functions. In this experiment, whether the administration of UC can reduce the reduction of hematopoietic and immune function associated with radiotherapy was explored.
[0088] Mice (body weight 24-27g) were irradiated with γ-rays at 60Co, 2Gy. UC2 was orally administered at 0.3 ml / kg for 30 days after irradiation. The mice were killed after 10 days, 20 days and 30 days of irradiation, and then the peripheral blood was extracted, and the number of red blood cells (RBC), white blood cells (WBC), and platelets (BP) was determined.
[0089] Table 6: Changes in the number of red blood cells (×10 12 / 1)(n=30)
[0090]
[0091] Table 7: Changes in platelet count (×10 9 / 1)(n=30)
[0092]
[0093] Table 8: Changes in the number of white blood ...
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