Method for producing animal feed based on cottonseed meal physical, chemical and microbial detoxification technology
A technology for animal feed and cottonseed meal, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of low detoxification rate and long fermentation period, and achieve the effects of simple operation, improved nutritional value and simple requirements.
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Embodiment 1
[0043] Embodiment 1: 40 mesh mixed powders were prepared according to the weight ratio of cottonseed meal: bran: corn flour=5:3:1. The ratio of compound mineral solution is 3g / L NaNO 3 , 0.5g / L MgSO 4 ·7H 2O, 4g / LK 2 HPO 4 , 0.2g / LNaCl, 1g / L FeSO 4 , 0.4g / L MnSO 4 and 0.1g / L CuSO 4 ·5H 2 O, use this composite mineral solution to adjust the humidity of the mixed powder, so that the ratio of material to water reaches 1:0.4. Steam sterilization at 80°C for 60 minutes. The cell concentration was 3.0×10 8 The cfu / ml Saccharomyces cerevisiae culture solution was added to the steam-treated mixed powder cooled to 30° C. with 10% inoculation amount, and fermented at 15° C. for 48 hours. Then press 1% access concentration to be 6.0×10 8 The Aspergillus oryzae spore liquid of cfu / ml continued to ferment for 60 hours at 15°C.
Embodiment 2
[0044] Embodiment 2: Prepare 40 mesh mixed powders according to the weight ratio of cottonseed meal: bran: corn flour=6:2:2. The ratio of compound mineral solution is 4g / L NaNO 3 , 2g / L MgSO 4 ·7H 2 O, 1g / LK 2 HPO 4 , 0.8g / L NaCl, 0.5g / L FeSO 4 , 0.1g / L MnSO 4 and 0.5g / L CuSO 4 ·5H 2 O, use this compound mineral solution to adjust the humidity of the mixed powder, so that the ratio of material to water reaches 1:0.9. Steam sterilization at 120°C for 20 minutes. The cell concentration was 4.6×10 8 The cfu / ml Saccharomyces cerevisiae culture solution was added into the steam-treated mixed powder cooled to 50° C. with 5% inoculum, and fermented at 38° C. for 12 hours. Then according to 7% access concentration is 3.5×10 8 The Aspergillus oryzae spore liquid of cfu / ml continued to ferment for 24 hours at 40°C.
Embodiment 3
[0045] Embodiment 3: Prepare 40-mesh mixed powder according to the weight ratio of cottonseed meal: bran: corn flour=5.5: 3.6: 1.8. The ratio of compound mineral solution is 5g / L NaNO 3 , 2.5g / L MgSO 4 ·7H 2 O, 3g / L K 2 HPO 4 , 0.5g / L NaCl, 1.5g / L FeSO 4 , 1g / L MnSO 4 and 0.6g / L CuSO 4 ·5H 2 O, use this composite mineral solution to adjust the humidity of the mixed powder, so that the ratio of material to water reaches 1:1, and steam sterilize at 100°C for 40 minutes. The cell concentration was 5.8×10 8 The cfu / ml Saccharomyces cerevisiae culture solution was added into the mixed powder cooled to 35° C. after steam treatment with 2% inoculation amount, and fermented at 20° C. for 45 hours. Then press 5% access concentration to be 4.5×10 8 The Aspergillus oryzae spore liquid of cfu / ml continued to ferment for 28 hours at 20°C.
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