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In-line test device and methods of use

A technology for fluid control devices and valve devices, which is applied in chemical instruments and methods, measuring devices, containers for laboratories, etc., and can solve problems such as low efficiency, expensive manufacturing materials, and complicated design and manufacturing.

Inactive Publication Date: 2010-04-14
雅培快速诊断国际无限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many of the above devices are not efficient at sample extraction by the collection device
In addition, many of these devices are complex to design and manufacture, and the materials used to make them are quite expensive

Method used

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  • In-line test device and methods of use
  • In-line test device and methods of use
  • In-line test device and methods of use

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0132] Example 1: Method of Use of Disease Screening Device: Streptococcus A (Strep-A)

[0133]Use standard man-made fiber swabs or polyester swabs to obtain throat samples from patients showing physical signs and pharyngitis symptoms. Swab the tonsil area of ​​the throat. Place the sample collection chamber of the test device on the test bench with a lateral flow test piece inside, put 4 drops of about 160 microliters of reagent A (2 molar sodium nitrate) and 4 drops of about 160 microliters of reagent B (0.2 molar Acetic acid) is added to the extraction unit. The swab containing the throat sample is inserted into the sample collection chamber and rotated repeatedly for approximately 10 seconds, after which the swab is incubated in the solution for 60 seconds. Thereafter, the valve structure is actuated while the swab remains in the sample collection chamber. The contents of the sample collection chamber, equivalent to approximately 200 microliters, were transferred to t...

Embodiment 2

[0134] Example 2: Method of using the disease detection device: Chlamydia

[0135] A rayon or polyester swab with a plastic stem, or a cytobrush is used to collect an endocervical sample. Keys on the sample collection chamber of the assay device are secured into corresponding keyholes located on the test bench, which is equipped with a lateral flow test strip device. Put 150 microliters of 1 equivalent potassium hydroxide into the sample collection chamber of the device, put the swab or cell brush into the container, rotate for 10-20 seconds and incubate for 5 minutes, after that, add 150 microliters of 1 molar acetic acid ( Containing 0.1% non-ionic active agent 20) into the container. Rotate the swab or cell brush for another 10-20 seconds. The valve mechanism is actuated and the swab or cell brush remains in the extraction device. Depending on whether a swab or a cell brush is used, approximately 150-250 microliters of the extraction vessel contents are filtered throug...

example 3

[0136] Example 3: Method of use of genetically modified crop inspection equipment: BTK protein

[0137] To determine whether cereal seeds or cereal crops have been genetically modified to produce Bacillus thuringiensis subsp. (BtK) protein, randomly select 5-10 grams of grain kernels from seed donors or from various grain ears. Thoroughly grind the sample to ensure homogeneity. Place a portion of the ground sample into the sample collection chamber of the assay device until the sample fills 3 / 4 of the volume of the extraction vessel. Add 500 µl of saline. The ground cereal and saline mixture was incubated for 2 minutes. Carefully transfer the sample collection chamber to the laboratory bench without spilling the contents. The key structure of the sample collection chamber is inserted into the corresponding key hole on the test platform, and the platform contains a lateral flow test piece device for detecting BtK protein. The valve unit is actuated to allow the liquid co...

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Abstract

The present invention recognizes that it can be desirable to have a sample receiving chamber integral to or engageable with a test platform, such as a test platform that includes a test strip. The sample receiving chamber is preferably separate or separable from the test platform, but that need not be the case. Preferably, a fluid flow actuating device or structure, such as a valve separates the sample receiving chamber from the test platform. The present invention provides such a device and methods of use.

Description

[0001] This application is a divisional application with the application number 02810170.7, the application date is April 29, 2002, and the invention title is "Assembly-type assay device and its use method". The parent application with application number 02810170.7 claims the rights of the previous U.S. utility model application No. 09 / 860,408, the application title is "In Line Test Device (In Line Test Device) and its method of use", filed on May 18, 2001, It is now included here for reference. technical field [0002] The present invention generally relates to assay devices including sample collection chambers and assay stations and methods of use thereof. Preferably, the sample collection chamber can be used to extract, prepare or dilute samples for analysis by the bench. The test station may include test units, such as test strips. The strip can be used as an analyte of interest, such as a disease state, medical condition, or pathogen. This application incorporates by r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N33/53G01N33/18G01N33/24G01N33/02B01L3/00
CPCB01L2300/0864B01L2200/027B01L2400/0644B01L2200/026B01L2300/0825B01L2300/0672B01L2400/0683B01L2200/025B01L2300/087B01L2400/0406B01L3/5023B01L2400/0677Y10T436/25375Y10T436/255
Inventor R·赫达克L·包蒂斯塔
Owner 雅培快速诊断国际无限公司
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