Method for preparing human-like collagen haemostatic sponge in scale

A technology of human-like collagen and hemostatic sponge, which is applied in medical science, absorbent pads, bandages, etc., can solve the problems of unsatisfactory hemostatic effect and poor mechanical properties, and achieve the effect of eliminating hidden dangers of viruses, low cost, and controllable degradation rate

Inactive Publication Date: 2010-04-21
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although both collagen and chitosan have good biocompatibility, the hemostatic material with collagen or chitosan alone has poor mechanical properties and unsatisfactory hemostatic effect, which cannot meet the requirements of clinical operations. After repeated tests, the invention was obtained ideal compound hemostatic material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Dissolve human-like collagen into a 1.0% solution with distilled water, and dissolve chitosan with a deacetylation degree of 70% and a molecular weight of 200,000 Daltons into a 1.0% solution with 1.0% acetic acid, and then mix the two by 5 : 1 (human-like collagen and chitosan dry weight mass ratio is (5 ~ 20): 1) mix, add 2.0% glycerin of the total amount of the solution, stir evenly, vacuum defoam after filtering, inject suitable shape After vacuum freeze-drying at -40°C, use 0.1% glutaraldehyde solution to cross-link at room temperature for 10 hours. After cross-linking, wash with distilled water for several times, vacuum freeze-dry again, seal and use Co-60 to extinguish Bacteria to obtain the finished product of human-like collagen hemostatic sponge.

Embodiment 2

[0027] Dissolve human-like collagen into a 2.0% solution with distilled water, and dissolve chitosan with a deacetylation degree of 95% and a molecular weight of 500,000 Daltons into a 0.5% solution with 0.5% acetic acid, and then mix the two by 10 : 1 (mass ratio) mixed, add 1.0% glycerol of the total amount of the solution, stir evenly, and use 0.5% glutaraldehyde solution to cross-link at 37°C for 20h after vacuum freeze-drying at -50°C. After the joint is completed, it is washed several times with distilled water, and then vacuum freeze-dried again to shape it. After sealing, it is sterilized with Co-60 to obtain a finished human-like collagen hemostatic sponge.

Embodiment 3

[0029] Dissolve human-like collagen into a 1.0% solution with distilled water, and dissolve chitosan with a deacetylation degree of 90% and a molecular weight of 300,000 Daltons into a 1.0% solution with 0.5% hydrochloric acid, and then mix the two by 15:1 (mass ratio) mixing, adding 1.2% sorbitol to the total amount of the solution, stirring evenly, and using 0.8% glutaraldehyde solution to cross-link at 45°C for 12h after vacuum freeze-drying at -50°C. After the joint is completed, it is washed several times with distilled water, and then vacuum freeze-dried again to shape it. After sealing, it is sterilized with Co-60 to obtain a finished human-like collagen hemostatic sponge.

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Abstract

The invention discloses a method for preparing human-like collagen haemostatic sponge in scale, which comprises the following steps: dissolving human-like collagen into 1.0 to 2.0 percent solution; dissolving chitosan into 0.5 to 1.0 percent solution by dilute acid; mixing the two solutions evenly, adding glycerin and/or sorbitol into the solution, mixing, stirring and filtering the solution, and then defoaming the solution in vacuum; injecting the mixed solution into a die, pre-freezing the mixed solution at a temperature of between 40 DEG C below zero and 80 DEG C below zero, cross-linking the mixed solution by using 0.1 to 1.0 percent glutaraldehyde solution after freezing and drying, washing the cross-linked product for several times by using distilled water after the cross-linking is finished and vacuum-freezing and drying the washed product again, and then sterilizing the product by Co-60 to obtain a finished product of the human-like collagen haemostatic sponge. Compared with the conventional haemostatic materials broadly applied in clinic, the prepared haemostatic sponge has good mechanical property and excellent biocompatibility, thoroughly stops inevitable virus hidden trouble of animal collagen haemostatic materials, and ensures the safety of the haemostatic material in clinical usage.

Description

technical field [0001] The invention relates to a method for large-scale preparation of a human-like collagen hemostatic sponge, which belongs to the field of biomedical materials. Background technique [0002] In wound first aid and surgical operations, wound bleeding and oozing are often encountered problems. A large amount of blood loss can be life-threatening. At the same time, blood is a natural microbial culture medium. Bleeding or oozing will increase the chance of infection, thereby reducing The success rate of surgery. A good hemostasis technique is the key to successful surgery, so surgical hemostasis is one of the cores of surgical techniques. Temporarily control bleeding, cover and bandage the wound as soon as possible, isolate the wound from the outside world to reduce the chance of infection, and create good wound conditions for debridement. Severe traffic injuries in normal times, firearm injuries in wartime, terrorist attacks, emergency rescue and disaster ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/42
Inventor 范代娣马晓轩朱晨辉段志广
Owner NORTHWEST UNIV(CN)
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