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Method for continuously extracting antibactial substances and antioxidant substances from pyrolignucs acid

A technology of antioxidant substances and antibacterial substances, applied in botany equipment and methods, chemical instruments and methods, antibacterial drugs, etc., can solve the problem of high water content, poor antioxidation effect, and inability to obtain antibacterial substances and antioxidant substances at the same time and other problems, to achieve the effect of simple method, enhanced antibacterial effect and easy operation

Inactive Publication Date: 2010-04-28
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the disadvantages that wood vinegar has high water content, antibacterial and antioxidative effects, and cannot obtain antibacterial substances and antioxidant substances during extraction in the above-mentioned prior art, the present invention provides a method for continuously extracting antibacterial substances from wood vinegar. and antioxidant substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Extraction of antibacterial substances and antioxidant substances

[0025] Extract the wood vinegar stock solution with ethyl acetate with a volume ratio of 0.5:1 until it is colorless, and use 0.6mol / L NaHCO to extract 3 The solution was extracted 3 times at a volume ratio of 1:1, and the combined NaHCO 3 Extract solution and with 3mol / LH 2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three times with ethyl acetate at a volume ratio of 0.5:1, and the obtained extract was distilled under reduced pressure at 40°C and a vacuum of 0.1MPa, and dried at normal pressure and 70°C. The obtained solid is the antibacterial substance. The above 0.6mol / L NaHCO 3 The remaining solution after extraction was treated with 0.6mol / L Na 2 CO 3 The solution was extracted 3 times at a volume ratio of 1:1, and Na 2 CO 3 Extract solution and with 3mol / LH 2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three...

Embodiment 2

[0028] Embodiment 2: Extraction of antibacterial substances and antioxidant substances

[0029] Extract the wood vinegar stock solution with ethyl acetate with a volume ratio of 1:1 until it is colorless, and use 0.7mol / L NaHCO to extract 3 The solution was extracted 3 times at a volume ratio of 1:1, and the combined NaHCO 3 Extract solution and with 3mol / LH2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three times with ethyl acetate at a volume ratio of 1:1, and the obtained extract was distilled under reduced pressure at 40°C and a vacuum of 0.1MPa, and dried at normal pressure and 70°C. The obtained solid is the antibacterial substance. The above 0.7mol / L NaHCO 3 The remaining solution after extraction was treated with 0.5mol / L Na 2 CO 3 The solution was extracted 3 times at a volume ratio of 1:1, and Na 2 CO 3 The extract was mixed with 3mol / L H 2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three tim...

Embodiment 3

[0032] Embodiment 3: Extraction of antibacterial substances and antioxidant substances

[0033] Extract the wood vinegar stock solution with ethyl acetate with a volume ratio of 0.3:1 until it is colorless, and use 0.5mol / L NaHCO to extract 3 The solution was extracted 3 times at a volume ratio of 1:1, and the combined NaHCO 3 Extract solution and with 3mol / LH 2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three times with ethyl acetate at a volume ratio of 0.3:1, and the obtained extract was distilled under reduced pressure at 40°C and a vacuum of 0.1MPa, and dried at normal pressure and 70°C. The obtained solid is the antibacterial substance. The above 0.5mol / L NaHCO 3 The remaining solution after extraction was treated with 0.4mol / L Na 2 CO 3 The solution was extracted 3 times at a volume ratio of 1:1, and Na 2 CO 3 Extract solution and with 3mol / LH 2 SO 4 The solution was adjusted to the pH value of the log vinegar, extracted three...

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Abstract

The invention discloses a method for continuously extracting antibactial substances and antioxidant substances from pyrolignucs acid; in the method, the pyrolignucs acid stock solution is extracted to be colourless by ethylacetate, extract liquid is extracted for at least three times with NaHCO3 with 1:1 volume ratio, the NaHCO3 extract liquid is mixed and the pH value of the mixed NaHCO3 extract liquid is adjusted to be the pH value of original pyrolignucs acid, and then the mixed NaHCO3 extract liquid is extracted for at least three times by ethylacetate with 1:1 volume ratio, pressure reduction distillation and drying are carried out to the obtained extract liquid to obtain solid which is the antioxidant substance, and the remaining solution is extracted for at least three times by Na2CO3 with 1:1 volume ratio, the Na2CO3 extract liquid is combined, and the pH value of the mixed Na2CO3 extract liquid is adjusted to be the pH value of original pyrolignucs acid, and the mixed Na2CO3 extract liquid is extracted for at least three times by dichloromethane, and the normal pressure distillation and drying are carried out to the extract liquid to obtain the solid which is the antioxidant substance. The antibactial substance and the antioxidant substance which are extracted have strong bacteriostasis and antioxidant effect compared with the pyrolignucs acid stock solution, therefore, the method can be widely applied to the field such as agriculture, food, daily health products and medicines.

Description

technical field [0001] The invention belongs to the field of natural product extraction, in particular to a method for continuously extracting antibacterial substances and antioxidant substances from wood vinegar, specifically extracting organic acids, phenols and the like from wood vinegar with an organic solvent. A mixture of organic substances with antibacterial and antioxidant activity, by adjusting the pH of the extract to change the existing form of antibacterial substances and antioxidant substances in the extract (conversion between ions and molecular forms), and then extract the antibacterial substances and antioxidant substances with organic solvents. A method of antioxidant substances. Background technique [0002] Wood vinegar is a reddish brown or dark brown liquid formed by dry distillation of woody materials (such as wood, wood processing waste or branches produced by forest tending and felling, etc.) after clarification and separation of precipitated wood tar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01N65/08A01P1/00A01P3/00C09K15/34A23L3/3472A61K36/00A61K36/52A61P31/04A61P31/10A61P39/06
Inventor 尉芹马希汉张珊珊刘胜臣薛雷施琳
Owner NORTHWEST A & F UNIV
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