Tryptophan premix
A technology of tryptophan and premix, applied in the field of feed, can solve problems such as poor feeding effect, livestock and poultry don't like to eat, insufficient palatability, etc., and achieve the effect of improving utilization rate
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Embodiment 1
[0028] The composition of the liquid culture medium of Escherichia coli is: glucose 30g L -1 , (NH 4 ) 2 SO 4 2.0g·L -1 , corn steep liquor 30mL, bean cake hydrolyzate 20mL, KH 2 PO 4 ·3H 2 O 1.0g·L -1 , MgSO 4 ·7H 2 O 0.4g·L -1 , MnSO 4 ·H 2 O 0.01g·L -1 , FeSO 4 ·7H 2 O 0.01g·L -1 , VB1300μg, VH200μg, pH value is controlled at 7.0-7.2. The sterilization pressure is 0.075Mpa, and the sterilization takes 15 minutes. Escherichia coli was cultured with shaking in the liquid medium for 16 hours, and the culture temperature was 36°C.
[0029] Escherichia coli is cultivated in a liquid medium to obtain mature seeds, and the mature seeds are then transferred to the sour medium in a fermenter for fermentation. The composition of the fermentation medium is: glucose 130g L -1 , (NH 4 ) 2 SO 4 40g·L -1 , phenylalanine Phe 0.15g·L -1 , Tyrosine Tyr 0.15g·L -1 , bean cake hydrolyzate 19mL, corn steep liquor 22mL, KH 2 PO 4 ·3H 2 O 10.0g·L -1 , MgSO 4 ·7H 2 O...
Embodiment 2
[0034] The difference between embodiment two and embodiment one is: before corn cob powder is added to the thick slurry, silicon dioxide and sodium chloride of feed grade are added in the thick slurry in agitated state, continue to stir for 4 minutes, Add 80-mesh corncob powder into the thick slurry in a state of stirring, and the addition amount of the corncob powder is 1 / 10 of the thick slurry to obtain a mixture with a tryptophan content of 4%. The parts by weight of silicon dioxide and sodium chloride in the mixture are 3%. The mixture was air-dried to control its water content at 1%.
Embodiment 3
[0036] The difference between embodiment three and embodiment one is: before corn cob powder is added to thick slurry, calcium carbonate, silicon dioxide and sodium chloride of feed grade are added in the thick slurry that is stirring state, continue to stir After 8 minutes, add 60 mesh corncob powder into the thick slurry in a stirring state, the amount of corncob powder added is 1 / 5 of the thick slurry to obtain a mixture with a tryptophan content of 2%. The parts by weight of calcium carbonate, silicon dioxide and sodium chloride in the mixture are 5%. The mixture was air-dried and dried so that its water content was controlled at 3%.
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