Biocompatible and temperature-sensitive nano composite hydrogel and preparation method thereof

A biocompatible and nanocomposite technology, applied in the field of biocompatibility and temperature-sensitive nanocomposite hydrogel and its preparation, can solve the problems of difficult to meet tissue engineering, poor mechanical comprehensive performance, poor biocompatibility, etc. , to achieve the effects of low cost, high biocompatibility and simple preparation method

Inactive Publication Date: 2010-05-12
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] From the above analysis, it can be seen that the biocompatible hydrogels currently prepared have limited sources of raw materials and no obvious temperature sensitivity; and the general temperature-sensitive hydrogels have poor mechanical properties due to the use of chemical cross-linking agent MBA. Poor; using the physical cross-linking agent Clay to prepare hydrogels with high mechanical properties, but its biocompatibility is poor, so it is difficult to meet the needs of practical applications such as tissue engineering

Method used

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  • Biocompatible and temperature-sensitive nano composite hydrogel and preparation method thereof
  • Biocompatible and temperature-sensitive nano composite hydrogel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Add 1.20g of isopropylacrylamide, 0.25g of laponite, and 0.05g of polyhydroxybutyrate into 10mL of deionized water in sequence, and in a sealed container at 20°C, pass nitrogen gas, ultrasonically disperse for 30min, and stir for 45min until complete Disperse to form a homogeneous solution; cool the above mixed solution to 3°C with ice cubes, add 0.01 g of ammonium persulfate after 30 minutes, and inject 20 μL of N,N,N'N'-tetramethylethylenediamine after 10 minutes, under nitrogen protection, Continue to stir for 10 minutes until the mixed solution begins to appear viscous; immediately pour the viscous mixed solution into a glass mold with a thickness of 2mm, and stand at 20°C for in-situ free radical polymerization for 24 hours, and then the reaction product Soak in deionized water for 2 weeks to obtain the biocompatibility and temperature-sensitive nanocomposite hydrogel.

[0040] The obtained nanocomposite hydrogel: the volume phase transition temperature is 33.2°C, ...

Embodiment 2

[0042] Add 1.0g of isopropylacrylamide, 0.2g of lithium magnesium silicate, and 0.3g of poly(hydroxybutyrate-hydroxyvalerate) into 10mL of deionized water in sequence, and in a sealed container at 20°C, blow nitrogen into it, and ultrasonically disperse 30min, stirred for 60min, until completely dispersed to form a uniform solution; the above mixed solution was cooled to 2°C with ice cubes, 0.02g ammonium persulfate was added after 30min, and 40μL of N,N,N'N'-tetramethylethane was injected after 10min. Diamine, under the protection of nitrogen, continue to stir for 10 minutes until the mixed solution begins to appear viscous; immediately pour the viscous mixed solution into a glass mold with a thickness of 2mm, and stand at 25°C for in-situ free radical polymerization After reacting for 20 h, the reaction product was soaked in deionized water for 2 weeks to obtain the biocompatible and temperature-sensitive nanocomposite hydrogel.

[0043] The obtained nanocomposite hydrogel: ...

Embodiment 3

[0045] Add 1.3g of diethylacrylamide, 0.15g of laponite, and 0.05g of poly-3-hydroxybutyrate-3-hydroxyhexanoate (PHBHHx) into 10mL of deionized water in sequence, and pass it into a sealed container at 20°C Nitrogen, ultrasonically disperse for 30 minutes, stir for 30 minutes until completely dispersed to form a uniform solution; cool the above mixed solution to 3°C with ice cubes, add 0.015g ammonium persulfate after 30 minutes, and inject 30 μL after 10 minutes N, N, N'N'- Tetramethylethylenediamine, under the protection of nitrogen, continue to stir for 10 minutes until the mixed solution begins to appear viscous; immediately pour the viscous mixed solution into a glass mold with a thickness of 2mm, and stand at 25°C for the original Then, the reaction product was soaked in deionized water for 2 weeks to obtain the biocompatible and temperature-sensitive nanocomposite hydrogel.

[0046] The obtained nanocomposite hydrogel: the volume phase transition temperature is 33.9°C, ...

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Abstract

The invention relates to a biocompatible and temperature-sensitive nano composite hydrogel and a preparation method thereof. The hydrogel contains alkyl acrylamide M1 with temperature-sensitive property, inorganic nano-powder M2 and polyhydroxyalkanoate M3 with good biocompatibility, and the mass percentage of the three is 94-60: 1-20: 5-39. The preparation method comprises the steps that: the M1, the M2 and the M3 are subjected to in-situ free radical polymerization reaction in aqueous solution, and the nano composite hydrogel with biocompatibility and temperature-sensitive property is obtained after soaking and impurity removal. The method has simple and easily controlled preparation process, and can implement automatic desorption of cells by reducing the temperature; the biocompatibility and the temperature-sensitive property of the hydrogel can be adjusted by changing the density of a crosslinker, the dosage of the M3 and the component; and the method can be widely applied in the fields of tissue engineering and the like.

Description

technical field [0001] The invention relates to the field of biocompatible and environment-sensitive polymer materials, in particular to a biocompatible and temperature-sensitive nanocomposite hydrogel and a preparation method thereof. The nanocomposite hydrogel can be applied in the fields of tissue engineering and the like. Background technique [0002] Hydrogel is a kind of hydrophilic cross-linked polymer between liquid and solid that can significantly swell in water and retain a large amount of water but cannot be dissolved in water. It can accommodate several times or even hundreds of times the weight of the polymer itself. of water. Environmentally sensitive hydrogels can sense small changes or stimuli in the external environment (such as temperature, pH, light, electricity, and pressure, etc.), and can produce corresponding changes in physical structure and chemical properties. In the change of external environment, due to the controllability and simplicity of temp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/52C12N5/00C12N5/09
Inventor 张青松董有玉赵义平陈莉
Owner TIANJIN POLYTECHNIC UNIV
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