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Chlorella sorokiniana CS-01 and culture method thereof for producing grease

A cultivation method and technology of chlorella, applied in the field of fermentation, can solve problems such as shortage of land resources and rising crop prices, and achieve the effects of good application potential, good growth and high yield

Inactive Publication Date: 2012-07-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But biodiesel produced from oil crops, waste cooking oil and tallow fatty acids can meet even a fraction of current vehicle fuel demand
At the same time, the use of plant raw materials has common problems in all countries: 1. Whether it is suitable for the local climate to achieve high and stable yields; 2. The problem of shortage of land resources during the planting process and the resulting increase in the price of other crops

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Under sterile conditions, single algae were picked from a solid plate and dropped into a small test tube containing 5 mL of sterilized culture solution, placed in an illuminated incubator for static culture, and grown to the end of the exponential phase with an inoculation ratio of 1:10 to expand the culture.

[0029] Inoculate the algae liquid into 3L sterilized BG11 medium with a final concentration of 1×10 6 A / mL~3×10 6 per mL, the culture temperature was 30°C, the pH of the medium was 8.1, the nitrogen source was sodium nitrate with a concentration of 0.5 g / L, and the concentration of EDTA-Fe(III) after adding was 1×10 -4 mol / L, nitrogen source and EDTA-Fe(III) were all added at one time, the light intensity was 10000LX, the light-dark ratio was 14:10, and the culture method was static culture for 30 days.

[0030] The algae cells were collected by centrifugation, freeze-dried in vacuum, weighed the dry weight of the algae powder and calculated the output of the al...

Embodiment 2

[0032] Under sterile conditions, single algae were picked from a solid plate and dropped into a small test tube containing 5 mL of sterilized culture solution, placed in an illuminated incubator for static culture, and grown to the end of the exponential phase with an inoculation ratio of 1:10 to expand the culture.

[0033] Inoculate the algae liquid into 3L sterilized BG11 medium with a final concentration of 1×10 6 A / mL~3×10 6 per mL, the culture temperature was 30°C, the pH of the medium was 11, the nitrogen source was sodium nitrate with a concentration of 0.5 g / L, and the concentration of EDTA-Fe(III) after adding was 1×10 -4 mol / L, the addition method is one-time addition, the light intensity is 10000LX, the light-to-dark ratio is 14:10, and the cultivation method is static cultivation for 30 days.

[0034] The algae cells were collected by centrifugation, freeze-dried in vacuum, weighed the dry weight of the algae powder and calculated the output of the algae powder t...

Embodiment 3

[0036] Under sterile conditions, single algae were picked from a solid plate and dropped into a small test tube containing 5 mL of sterilized culture solution, placed in an illuminated incubator for static culture, and grown to the end of the exponential phase with an inoculation ratio of 1:10 to expand the culture.

[0037] Inoculate the algae liquid into 3L sterilized BG11 medium, and the final concentration after inoculation is 1×10 6 A / mL~3×10 6 per mL, the culture temperature was 30°C, the pH of the medium was 8.1, the nitrogen source was sodium nitrate with a concentration of 0.2 g / L, and the concentration of EDTA-Fe(III) after adding was 1×10-4 mol / L, EDTA-Fe(III) is added in one time. On the seventh day of cultivation, sodium nitrate was fed (the original nitrogen source was basically completely consumed), the feeding amount was 0.2 g / L culture solution, the light intensity was 10000 Lx light-dark ratio was 14:10, and the culture method was aeration culture for 20 days...

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Abstract

The invention discloses chlorella sorokiniana CS-01 and a culture method thereof for producing grease. The preservation number of the chlorella is CCTCC M209220. The culture method for producing the grease comprises the following steps: inoculating index-cultured strains to BG11 sterilized culture solution, adding 0.3 to 1.5 g / L of sodium nitrate or urea and 1*10-5 mol / L to 1*10-3 mol / L of EDTA-Fe (III) into the culture solution, culturing the strains by ventilating or without ventilating at the temperature of between 25 and 35 DEG C, pH of 6 to 11, 6,600 to 10,000 Lx and lighting ratio of 14:10, and harvesting the grease, wherein the dry weight of the harvested cells is as high as 970 mg / L, and the content of the grease can reach 57 percent. The strain is fresh-water unicellular chlorella, has the advantages of strong environmental adaptation, high growing speed and higher grease yield, and has potential serving as a biodiesel preparation raw material to be cultured in a large scale in interior regions.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a chlorella (Chlorellasorokiniana) CS-01 and a method for cultivating and obtaining oil. Background technique [0002] Due to the intensification of the contradiction between the increasing demand for fossil fuels and the reduction of their reserves, and the emission of various pollutants from the use of fossil fuels, it is widely recognized that the continued use of petroleum fuels is impossible. Renewability of transportation fuels and zero emission of air pollutants are important for sustainable economic and environmental development. Biodiesel is a proven fuel. The technology to produce and use biodiesel has existed for more than 50 years. Currently, biodiesel is mainly produced from vegetable and animal fatty acids rather than microalgae. In the United States, biodiesel is primarily derived from soybean oil, with other sources including palm oil, canola o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/13Y02E50/10
Inventor 夏金兰邱冠周王润民李丽万民熙刘鹏黄斌聂珍媛
Owner CENT SOUTH UNIV
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