Bacillus sp. E2-Y with 17 beta-estradiol degrading ability and application thereof

A Bacillus, E2-Y technology, applied in the field of biological treatment of environmental pollutants, can solve problems such as Bacillus that have not yet been found, and achieve the effect of broad application prospects

A Bacillus, E2-Y technology, applied in the field of biological treatment of environmental pollutants, can solve problems such as Bacillus that have not yet been found, and achieve the effect of broad application prospects

CN101705195AActive Publication Date: 2010-05-12ZHEJIANG UNIV OF TECH
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  • Bacillus sp. E2-Y with 17 beta-estradiol degrading ability and application thereof
  • Bacillus sp. E2-Y with 17 beta-estradiol degrading ability and application thereof
  • Bacillus sp. E2-Y with 17 beta-estradiol degrading ability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation, purification and identification of Bacillus sp.E2-Y

[0020] Bacillus sp.E2-Y is a Gram-positive bacterium domesticated and isolated from the activated sludge of a pharmaceutical factory wastewater treatment station in Xianju, Zhejiang. Specific steps are as follows:

Embodiment 2

[0023] Example 2: Detection of Bacillus sp.E2-Y on E2 degradation performance

[0024] 1. To investigate the degradation ability of E2-Y to different initial concentrations of E2.

[0025] At a concentration of 0.5-50mg / L, strain E2-Y can degrade E2, but the degradation rate is different. It can completely remove E2 with an initial concentration of ≤10mg / L within 5 days, and can remove the initial concentration of ≤50mg / L in 7 days The E2 is completely removed. The specific steps are as follows:

[0026] 1) R stored at 4°C with an inoculation loop 2 Pick a ring of bacteria on the slant of medium A, and connect it to a sterile container filled with 100mL R 2 In the Erlenmeyer flask of A liquid medium, cultivate at 30° C., 150 r / min shaker for 24 hours to logarithmic growth phase. Then centrifuge at 10000r / min for 10min, pour off the supernatant, add sterile inorganic salt liquid culture medium without E2 to wash, then centrifuge at 10000r / min for 10min, pour off the supernat...

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Abstract

The invention provides an effective Bacillus sp. with strong endurance capacity and E2 degrading ability and the bacillus is conserved in the China Center for Type Culture Collection; the conservation address is Wuhan University, the postal code is 430072, the CCTCC NO is M 209150, and the conservation date is July 15, 2009. The Bacillus sp. E2-Y is selected from a wastewater treatment unit, is the dominant strain in the wastewater treatment system and can adapt to complicated actual conditions, thus having wide application prospects.

Description

(1) Technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a new bacterial strain with 17β-estradiol degradation ability—Bacillus sp. E2-Y and its application. (2) Background technology [0002] Environmental Estrogens are environmental chemicals that adversely affect the maintenance of normal homeostasis, reproduction, growth and behavior of biological organisms. Even if there is only a small amount of such substances in the environment, it may lead to a decrease in the secretion of sex hormones in various organisms, weakened activity, decreased sperm count, abnormal reproductive organs, increased incidence of cancer, etc., and affect the reproductive system and immune system of various organisms and nervous system etc. Environmental estrogens can be roughly divided into natural estrogens (17β-estradiol, estrone, estriol, etc.), phytoestrogens and fungal estrogens, and synthetic ...

Claims

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Application Information

Patent Timeline
12 May 2010
Publication
CN101705195A
IPC
C12N1/20; A62D3/02; C02F3/34; C02F101/34; C02F101/30; A62D101/28; A62D101/20; C12R1/07
Inventors
姜理英; 陈建孟