Method for extracting black fungus polysaccharide

A black fungus polysaccharide and extraction method technology are applied in the extraction field of black fungus polysaccharide, which can solve the problems of reducing bioavailability, easily destroying polysaccharide structure, etc., and achieve the effects of stable and fast ultrafiltration rate, shortening process time and low crushing cost.

Inactive Publication Date: 2010-05-26
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional black fungus polysaccharide extraction uses water extraction and alcohol precipitation, and the polysaccharide is difficul

Method used

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  • Method for extracting black fungus polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1 Materials and reagents

[0027]Black fungus (provided by Zhejiang Fulaisen Green Industry Group Co., Ltd.); absolute ethanol, concentrated sulfuric acid, glucose, and phenol, all of analytical grade.

[0028] 2 experimental equipment

[0029] UV-2000 ultraviolet spectrophotometer; HMB-701 ultrafine pulverizer; HELDS / BF laser particle size analyzer; RE-52A rotary evaporator; WVD-01 microwave vacuum drying oven.

[0030] 3 Experimental methods

[0031] 3.1 Black fungus crushing process

[0032] Get the black fungus of 200g, after ordinary mechanical coarse pulverization, sieve with 40 mesh standard sieves earlier, then adopt superfine pulverizer to pulverize under-sieve material, regulate and control superfine pulverizer fan speed to be 16000r / min, obtain black fungus Fungus superfine powder.

[0033] 3.2 Determination of particle size of dry powder obtained by superfine grinding of black fungus

[0034] Use the HELDS / BF laser particle size analyzer, adopt the VIBR...

Embodiment 2

[0051] The experimental operation is the same as that in Example 1, except that 20 g of black fungus ultrafine powder is added, 2000 g of water is added, and the temperature is 80 ° C. It is extracted with 18 KHz ultrasonic waves for 30 min, extracted twice, centrifuged, and combined to obtain the above two extractions. The supernatant liquid obtained was 1890ml of combined supernatant, and other operations and conditions were the same as in Example 1 to obtain 2.95g of black fungus polysaccharide, with a polysaccharide content of 43.18%, and the dissolution rate of black fungus polysaccharide was 14.75%.

Embodiment 3

[0053] The experimental operation is the same as that in Example 1, except that 20 g of black fungus superfine powder is added, 600 g of water is added, and the temperature is 40 ° C. It is extracted with 22 KHz ultrasonic waves for 50 min, extracted twice, centrifuged, and combined to obtain the above two extractions. Supernatant liquid obtained 530ml of combined supernatant, and other operations and conditions were the same as in Example 1 to obtain 2.59g of black fungus polysaccharide, with a polysaccharide content of 42.18%, and the dissolution rate of black fungus polysaccharide was 11.96%.

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Abstract

The invention provides a method for extracting black fungus polysaccharide, which comprises the following steps of: (1) taking dry black fungi, mechanically pulverizing the dry black fungi coarsely, screening the pulverized black fungi with a 40-mesh sieve, then pulverizing the black fungi again by an ultrafine pulverizer, and adjusting and controlling the rotating speed of a fan of the ultrafine pulverizer to between 10,000 and 22,000 r/min to obtain ultrafine powder of the black fungi; mixing the ultrafine powder of the black fungi and water in a mass ratio of 1:20-100; extracting the mixture for 20 to 90 minutes by using 10 to 40 kHz ultrasonic waves at the temperature of between 40 and 80 DEG C; centrifugating extracting solution to obtain a supernatant fluid and a deposit; adding the supernatant fluid into a mixture of diatomite and activated carbon in a mass ratio of the diatomite to the activated carbon of 1 to 3, wherein the added amount of the mixture is 2 to 8 percent of the weight of the supernatant fluid; fully stirring the mixture, than then filtering the mixture; performing membrane filtration on the filtrate by using a 50kD polysulphone membrane; and collecting macromolecular concentrated solution which is not filtered through the membrane, and drying the macromolecular concentrated solution to obtain the black fungus polysaccharide. The prepared black fungus polysaccharide has high purity and high bioactivity.

Description

(1) Technical field [0001] The invention relates to a method for extracting black fungus polysaccharide. (2) Background technology [0002] Black fungus Auricularia auricula (L.exHook.) Underwood, also known as light fungus, fine fungus, cloud ear, is a large fungus, belonging to Basidiomycetes, Auricularia, Agaricaceae, Auricularia. The polysaccharides extracted from the fruiting body of black fungus are composed of fucose, mannose, glucose and glucuronic acid, including β-(1→3), β-(1→6) and α-(1→3) Several types of glycosidic bonds, molecular weight in 1×10 5 Da~2×10 5 Da between. A large number of medical and pharmacological studies have shown that black fungus polysaccharides have anti-platelet aggregation, anti-tumor, anti-aging, antibacterial, hypoglycemic, immune function regulation, anti-ulcer, anti-hepatitis and anti-mutation, hypolipidemic and promote serum protein biosynthesis and other effects. Such as Li Wenning (2003) in the patent "preparation method of b...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张拥军何杰民李佳
Owner CHINA JILIANG UNIV
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