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Application of metabonomic technology in intrauterine growth retardation

A technique for intrauterine growth retardation and metabolomics, which is applied in the fields of analysis, material testing, and biological testing using nuclear magnetic resonance, and can solve problems such as the application of metabolomics technology that has not been seen.

Inactive Publication Date: 2010-05-26
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, there is no report on metabolomics research related to early prenatal diagnosis, and no metabolomics technology has been found in the application of intrauterine growth retardation or similar reports at home and abroad.

Method used

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  • Application of metabonomic technology in intrauterine growth retardation
  • Application of metabonomic technology in intrauterine growth retardation
  • Application of metabonomic technology in intrauterine growth retardation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Establishment of Intrauterine Growth Retardation Model Induced by Nicotine and Caffeine

[0036] Implementation process:

[0037] Experimental method: Rats were caged at 6PM every day with a ratio of male to female of 2:1. The female rat's vaginal plug and microscopic examination were observed in the next morning. The day when the female rat's vaginal plug or sperm was found was regarded as day 0 of conception. The pregnant rats were randomly divided into 3 groups: normal control group, nicotine group, and caffeine group, with 10 rats in each group. Starting from the 12th day of pregnancy, they were given nicotine 2 mg·kg respectively. -1 d -1 Subcutaneous injection, the caffeine group was given caffeine 180mg·kg -1 d -1 Gastrointestinal administration, the normal control group was given an equal volume of distilled water. On the 20th day of pregnancy, the pregnant mice were first anesthetized with isoflurane, and then part of the blood was collected throu...

Embodiment 2

[0042] Example 2 Application of NMR Metabolomics Technology in Intrauterine Growth Retardation Model Rats

[0043] (1) Animal treatment and material collection: Refer to Example 1 for the methods of pregnancy, administration and treatment of rats. On the 20th day of pregnancy, the pregnant mice were first anesthetized with isoflurane, and then part of the blood was collected through the carotid artery, anticoagulated with heparin, and plasma was prepared. Biological samples (including maternal blood, fetal blood and amniotic fluid) were collected from normal pregnancy and caffeine-induced intrauterine growth retardation model rats at different gestational periods, prepared plasma, quick-frozen in liquid nitrogen, and stored at ultra-low temperature (-78°C--82°C). ℃) Stored in the refrigerator for testing.

[0044] (2) NMR spectrometry: the plasma sample was made into physiological saline phosphate buffer solution containing sodium and potassium so that the concentration was 3...

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Abstract

The invention relates to application of metabonomic technology in intrauterine growth retardation, which is mainly characterized by starting from the view of system biology (Cowley AW Jr.The elusive field of systems biology. Physiol Genomics.2004;16(3):285-286), and applying a magnetic resonance spectroscopy technology based metabonomic method to seek a nuclear magnetic resonance spectrogram capable of specifically reflecting the intrauterine growth retardation in the peripheral blood of an experimental pregnant animal or a pregnant woman so as to provide proofs for further diagnosing the intrauterine growth retardation. The application comprises the following steps: during analysis, detecting a biological sample (comprising parent blood, embryo blood and amniotic fluid) of the pregnant animal or the pregnant woman in normal pregnancy and intrauterine growth retardation experiment by using nuclear magnetic resonance technology, acquiring a metabonomic spectrogram, analyzing the metabonomic spectrogram by using a pattern identification method, determining the metabonomic change of the intrauterine growth retardation through analysis and comparison, and acquiring one or more (groups of) nuclear magnetic resonance spectrograms for reflecting the intrauterine growth retardation from the metabonomic change so as to provide theoretical and experimental proofs for the subsequent further determination of a biomarker and the application of the metabonomic technology in the intrauterine growth retardation. The application has the advantages of high sensitivity, good specificity, early stage, non-invasion or micro-invasion and the like.

Description

technical field [0001] The present invention relates to the field of medical medicine technology, and more specifically relates to the application of a nuclear magnetic resonance-based metabolomics technology in the detection of intrauterine growth retardation in experimental animals or humans, and is expected to be extended to the use of metabolomics methods to confirm biomarkers, Detection of intrauterine growth retardation in experimental animals or human fetuses. Background technique [0002] The present invention relates to the application of nuclear magnetic resonance-based metabolomics technology in the detection of experimental animals or human intrauterine growth retardation, and is mainly used in the prenatal early diagnosis laboratories of various medical research units and the research on prenatal early diagnosis in various laboratories . Application of NMR-based metabolomics technology in the detection of intrauterine growth retardation in experimental animals ...

Claims

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Application Information

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IPC IPC(8): G01N24/08G01N33/48
Inventor 汪晖冯江华唐惠儒梁赅刘岩松鄢友娥李小军张本坚
Owner WUHAN UNIV
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