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Preparation method of direct antihuman globulin reagent card

A technology of anti-human globulin and reagent card, applied in the medical field, can solve problems such as uncertainty, reduced sensitivity, large particles, etc., and achieve the effect of strong buffer capacity

Active Publication Date: 2012-12-12
JIANGYIN LIBO MEDICINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At home and abroad, there is no direct anti-human globulin test card produced by any manufacturer, and only some manufacturers have produced similar products, which can only detect antibodies that have sensitized red blood cells, but cannot determine whether the antibodies are IgG or C 3 class d
And the gel it adopts is dextran gel, the particle size of this gel is more than 70 nanometers, the particles are larger, and the gap between the gels is larger, so that the sensitivity of the test is reduced

Method used

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  • Preparation method of direct antihuman globulin reagent card

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Experimental program
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Embodiment 1

[0040] Step 1, preparation of gel suspension medium

[0041] Described gel suspending medium formula is as follows:

[0042] Methylparaben (5.5-6.5)×10 -4 g / ml

[0043] Propylparaben (1.0-1.5)×10 -4 g / ml

[0044] Glycine (1.6-1.9)×10 -2 g / ml

[0045] Sodium chloride (1.7-1.8)×10 -3 g / ml

[0046] Potassium dihydrogen phosphate (2.1-2.4)×10 -4 g / ml

[0047] Disodium hydrogen phosphate (4.6-4.8)×10 -4 g / ml

[0048] Bovine serum albumin ≤2%,

[0049] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.

[0050] Step 2. Preparation of the gel

[0051] Polyacrylamide dextran gel is selected, and the particle size is 30-60 nanometers. Soak in the gel suspension medium prepared in step 1, then wash with the gel suspension medium for 3-5 times, remove the broken fragments of the gel and the aggregated gel particles, and collect the applicable gelatin powder with uniform particle size and complete spherical shape. gel.

[0052] Step 3. Antib...

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Abstract

The invention relates to a preparation method of a direct antihuman globulin reagent card. The preparation method is characterized in that the reagent card comprises six microcolumn gel tubes respectively comprising two gel tubes containing anti-IgG, two gel tubes containing anti-C3d and two gel tubes containing gel suspension mediums, and each reagent card is shared by two persons. The method comprises the technical processes of preparation of the gel suspension mediums, preparation of gel, selection of antibody as well as preparation and split charging of gel. The direct antihuman globulin reagent card prepared by the method has high sensitivity, good specificity and stable quality, red blood corpuscles having sensitization to IgG antibodies and the red blood corpuscles having sensitization to C3d alexin can be detected by using the same card at the same time, and the red blood corpuscles having sensitization to IgG antibodies or the C3d alexin can be accurately determined.

Description

(1) Technical field [0001] The invention relates to a micro-column gel card for detecting incomplete antibody and (or) complement of sensitized red blood cells and its application. More specifically, the present invention relates to the composition, preparation method and application of a direct antiglobulin reagent card, and relates to medical fields such as blood testing. (2) Background technology [0002] The antiglobulin test was born in 1945. The blood of some patients contains agglutinins against the patient's red blood cells, but no agglutination reaction occurs after mixing these serums with red blood cells, and when serum antibodies are added, anti-human globulin reagents (anti-IgG), anti-C 3 d reagent, agglutination reaction occurs. This test is called the antiglobulin test. Initially only used to detect antibodies in serum, the same principle was later used to detect antibodies and complement coated red blood cells in vivo. At present, the direct antihuman glo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/68G01N33/80
Inventor 陈玉平钱国强朱庆平
Owner JIANGYIN LIBO MEDICINE BIOTECH
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