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A kind of preparation method of lentinan ester

A technology of lentinan and temperature control, which is applied in the field of preparation of lentinan esters, can solve the problems of high cost and unfavorable industrialization, and achieve the effects of low production cost, less investment in equipment and mild reaction conditions

Active Publication Date: 2011-12-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this document, although the modification reaction is not intense, the method uses enzyme catalysis, which increases the cost and is not conducive to industrialization

Method used

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  • A kind of preparation method of lentinan ester
  • A kind of preparation method of lentinan ester
  • A kind of preparation method of lentinan ester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 15.5g of lentinan powder in a round bottom flask, add 31.5ml of acetic acid into it, stir and shake well, then add 5 drops of concentrated sulfuric acid into it, set up the device on a multifunctional stirrer, and then stir and react at room temperature for about 20 hours.

[0025] After adding 2.0 g of sodium acetate white crystals into the round bottom flask, the stirring reaction was started. After about 4 hours, set up the device for distillation, heat it with an oil bath water pump to evacuate the acetic acid, and start to distill the liquid when the oil bath temperature is 60°C. After ten minutes, distill at a temperature of about 78°C. Vacuum until a tan dry solid is obtained, take out the solid in a round-bottomed flask, add enough dichloromethane (about 150 ml) to soak and wash it, and let it stand for 10 hours. Afterwards, the solution was suction filtered to obtain a filter residue and a filtrate, and the filtrate was evaporated on a rotary evaporator to...

Embodiment 2

[0028] The stirring reaction time of lentinan and acetic acid at normal temperature is about 12 hours, and other reaction conditions are the same as in Example 1. The infrared analysis spectrum of the obtained product is as follows: figure 2 As shown, the results show that the polysaccharide has a certain degree of esterification at this time.

Embodiment 3

[0030] The ratio of substrate lentinan and acetic acid is about 1g: 4ml, and the ratio of acetic acid: concentrated sulfuric acid is 6ml: 1 drop. Other reaction conditions are the same as in Example 1. The infrared analysis spectrum of the obtained product is as follows: Figure 5 As shown, the results show that the polysaccharide has a certain degree of esterification at this time.

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Abstract

The invention discloses a preparation method of lentinan ester, comprising the following steps: uniformly mixing lentinan and short-chain fatty acid, adding concentrated sulfuric acid as a catalyst, stirring at 20-30°C for 15-20 hours, and then adding raw material short-chain fatty acid The alkali metal salt neutralizes the remaining sulfuric acid. After the reaction is complete, control the temperature below 80°C and distill under reduced pressure until the solid crude product is obtained. The obtained solid crude product is extracted with an extractant, and the obtained filtrate is filtered and the temperature is controlled below 80°C. Evaporate to dryness to obtain shiitake mushrooms polysaccharide esters. The new preparation method provided by the invention has mild reaction conditions, low production cost, less equipment investment, and is easy to realize industrialization; and the lentinan ester prepared by the invention can maintain the primary structure without being destroyed, and can change the spatial conformation of the polysaccharide, Make it fully effective; greatly improve its antiviral and other activities, which is expected to make a breakthrough in the prevention and treatment of harmful viruses.

Description

technical field [0001] The invention relates to a preparation method of lentinan ester, in particular to an esterification method of short-chain organic acid and lentinan. Background technique [0002] Molecular modification and structural modification of polysaccharides are of great significance to improve the biological activity of lentinan. Existing experiments have proved that the modified lentinan has good biological activity, among which esterification is a very effective structure modification method, and the esterified lentinan generally has good antiviral activity. Esterification methods include sulfation, phosphorylation, acetylation, alkylation, sulfonylation, carboxymethylation, etc., and the most studied is sulfated polysaccharides. The method of introducing sulfate to polysaccharides depends on the type of main chain sugar unit. For furan type sugar units, the Nagasava method is generally used; Amide solvent method, the former application is more. Although t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 杨胜利钱俊青沈润溥
Owner ZHEJIANG UNIV OF TECH