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Method for microencapsulating cholesterol-degrading lactic acid bacteria

A cholesterol-lowering, microencapsulation technology, applied in the directions of microcapsules, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high cost, low intestinal localization release, low storage stability, etc.

Inactive Publication Date: 2012-05-09
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the traditional microencapsulation method of lactic acid bacteria is not completely suitable for cholesterol-lowering lactic acid bacteria. This type of method has low intestinal localization release, low storage stability, and high cost

Method used

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  • Method for microencapsulating cholesterol-degrading lactic acid bacteria
  • Method for microencapsulating cholesterol-degrading lactic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Activation of cholesterol-lowering lactic acid bacteria

[0062] Lactobacillus casei subsp. casei (L.Casei subsp.casei), which is a cholesterol-lowering lactic acid bacterium to be treated, isolated and purified through the improved KENJI selective medium (containing 0.04% bromothymol blue), was inoculated in the culture medium containing 0.75wt% CaCO 3 MRS slant medium was stored in a refrigerator at 4°C.

[0063] (1) Pick the colonies on the slant solid medium, perform gradient dilution (such as 1:10, 1:100, 1:1000, 1:10000...), and add the diluted solution to the MRS liquid medium to mix the bacteria , cultured at 37°C for 48 hours, and repeated the above operation 2 to 3 times;

[0064] (2) Pick the single colony and observe it under a Gram staining microscope until a pure culture is obtained.

[0065] The described Lactobacillus casei subsp. casei (L.Casei subsp.casei) is a strain with high cholesterol degrading ability screened by the following method, but t...

Embodiment 2

[0113] 1. Activation of cholesterol-lowering lactic acid bacteria

example 1

[0115] 2. Preparation of immobilized cholesterol-lowering lactic acid bacteria

[0116] (1) After activation, the bacterial liquid was centrifuged at 3000rpm / min for 10 minutes, and the supernatant was removed;

[0117] (2) Wash the collected bacteria with sterile PBS washing solution (pH 7.0), and centrifuge again. repeat three times;

[0118] (3) Resuspend the bacteria with a sterile PBS buffer solution of pH 7.0 at a volume ratio of 1:5 bacteria and buffer;

[0119] (4) Slowly pour 5mL of the suspended bacteria liquid into 1.5g of activated diatomaceous earth, while stirring continuously, and let stand for 2h;

[0120] (5) Suction filtration after the diatomite fully adsorbs the cells.

[0121] 3. Preparation of microcapsules by W / O / W complex emulsion method

[0122] (1) Disperse 1.0 g of diatomite containing cholesterol-lowering lactic acid bacteria into 35 mL of the inner layer water phase (3.5 wt% gelatin, 2 wt% sodium alginate);

[0123] (2) Add the aqueous phase s...

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Abstract

The invention discloses a method for microencapsulating cholesterol-degrading lactic acid bacteria, which comprises the steps of the activation of the cholesterol-degrading lactic acid bacteria, the preparation of immobilized cholesterol-degrading lactic acid bacteria, the preparation of microcapsules by a W / O / W multiphase emulsion method, and freeze-drying. The cholesterol-degrading lactic acid bacteria microcapsules prepared by adopting the multiphase emulsion method (W / O / W) have the characteristics of stability, non-toxicity and the like due to the fact that wall materials thereof all belong to natural polymer materials; the release rate of the microcapsules in intestinal juice within 2h is over 87.4 percent, and the viable count can reach 7.03*109cfu / mL; the viable count can still reach 6.81*106cfu / mL after the microcapsules are placed for 3h at the temperature of 50 DEG C, and can still reach 6*108cfu / mL after the microcapsules are stored for 30d; in vitro cholesterol degradation tests, the degradation rate of cholesterol is over 36.07 percent; and the microcapsules have better acid resistance, enteric property, high temperature resistance and cholesterol degradation, and the yield is about 86.62 percent. Capsule materials and carrier materials have low cost, so the cost is reduced.

Description

technical field [0001] The invention relates to a microencapsulation method of cholesterol-lowering lactic acid bacteria. Background technique [0002] There are hundreds of species of bacteria living in the human intestine, and the number exceeds one billion billion. Among them, beneficial bacteria to human health are represented by lactic acid bacteria and bifidobacteria. The probiotic effect of lactic acid bacteria has been recognized by the medical profession. Long-term research results show that lactic acid bacteria are very important to human health and longevity. According to research data in recent years, lactic acid bacteria, as beneficial bacteria colonizing the intestinal tract, have a variety of health effects: they can maintain micro-ecological balance and intestinal function, improve liver function, lower cholesterol, enhance immune function and anti-tumor effects. Lactic acid bacteria are abundant in yoghurt and other related fermented dairy products. In th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61P3/06C12N1/20C12N11/10A61K35/74C12R1/245C12N11/04A61K35/744
Inventor 侯红漫陈金子张公亮
Owner DALIAN POLYTECHNIC UNIVERSITY
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