Halogenohydrin dehalogenase mutant strain, halogenohydrin dehalogenase mutant and preparation method and application thereof

A technology of halohydrin dehalogenase and mutant, applied in the field of microorganisms, can solve the problem of not finding industrialized production of halohydrin dehalogenase and the like
CN101760468AActive Publication Date: 2010-06-30ANGELYEAST CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANGELYEAST CO LTD
Publication Date
2010-06-30

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Abstract

The invention provides a gene engineering bacteria for producing dehalogenase and realizes the industrial production of dehalogenase industrialised by culturing the gene engineering bacteria via fermentation technology. The invention also provides the use of the gene engineering bacteria; and the halogenohydrin dehalogenase produced by the gene engineering bacteria can be applied to ring-opening reaction of ortho-halogenohydrin dehalogenation epoxidation and epoxide.
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Description

technical field

[0001] The invention relates to the field of microorganisms, in particular to a halohydrin dehalogenase gene mutant, the construction, production and application of a genetically engineered bacterium for producing the halohydrin dehalogenase mutant. Background technique

[0002] Epoxides are recognized as one of the most important and widely used synthetic intermediates in organic synthesis. Such compounds are easy to prepare by chemical synthesis, but the stereoselectivity of the chemical reaction is poor. In the process of dehalogenation and epoxidation for the hydroxyl group and halogen linked to the chiral carbon, the chemical reaction generally has no stereoselectivity, although it can be used with the help of some chemical catalysts. Stereoselective reactions are carried out under the following conditions, but the general reaction conditions are harsh and the results are not ideal. With the help of the use of halohydrin dehalogenase biocatalyst, high s...

Claims

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