Homocysteine diagnosis/determination reagent (kit) and homocysteine concentration determination method

A technology of homocysteine ​​and cysteine, which is applied in the field of homocysteine ​​diagnosis/measurement reagents and the determination of homocysteine ​​concentration, which can solve the problems of complex operation, large variation of test data, and long test time, etc. question

Inactive Publication Date: 2010-06-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its characteristics are similar to those of high-performance liquid chromatography, but it ha

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 500mmol / L

[0054] Coenzyme 3mmol / L

[0055] Glutathione Homocystine Transhydrogenase 10000U / L

[0056] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0057] Lovastatin Nine Ketone Synthase 12000U / L

[0058] Glutathione disulfide 5mmol / L

[0059] Coenzyme A-Glutathione 5mmol / L

[0060] Dihydromonascus L 5mmol / L

[0061] Sodium bicarbonate 15mmol / L

[0062] S-Adenosyl Homocysteine ​​5mmol / L

[0063] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0064] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test subwavelength 405nm, v...

Embodiment 2

[0067] The homocysteine ​​diagnosis / measurement reagent of this embodiment is a dual reagent, including:

[0068] Reagent 1

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 50mmol / L

[0071] Coenzyme 3mmol / L

[0072] Glutathione disulfide 5mmol / L

[0073] Coenzyme A-Glutathione 5mmol / L

[0074] Dihydromonascus L 5mmol / L

[0075] Sodium bicarbonate 15mmol / L

[0076] S-Adenosyl Homocysteine ​​5mmol / L

[0077] Reagent 2

[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 50mmol / L

[0080] Glutathione Homocystine Transhydrogenase 10000U / L

[0081] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0082] Lovastatin Nine Ketone Synthase 12000U / L

[0083] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0084] Set on the automatic biochemical analyzer: temperature 37°C, reacti...

Embodiment 3

[0087] The homocysteine ​​diagnosis / measurement reagents of this embodiment are three reagents, including:

[0088] Reagent 1

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 50mmol / L

[0091] Coenzyme 3mmol / L

[0092] Glutathione disulfide 5mmol / L

[0093] Coenzyme A-Glutathione 5mmol / L

[0094] Dihydromonascus L 5mmol / L

[0095] Sodium bicarbonate 15mmol / L

[0096] S-Adenosyl Homocysteine ​​5mmol / L

[0097] Reagent 2

[0098] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0099] Stabilizer 500mmol / L

[0100] Glutathione-coenzyme A-glutathione transhydrogenase 12000U / L

[0101] Lovastatin Nine Ketone Synthase 12000U / L

[0102] Reagent 3

[0103] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0104] Stabilizer 500mmol / L

[0105] Glutathione Homocystine Transhydrogenase 10000U / L

[0106] After all the reagents are dissolved and prepared, they are divided into bottles to mak...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a homocysteine diagnosis/determination reagent (kit) using enzyme-colorimetry and enzyme-linked method technology. Meanwhile, the invention also relates to a homocysteine concentration determination method, reagent compositions and components, which belong to the technical filed of medical test and determination. The reagent (kit) of the invention mainly includes: buffer solution, coenzyme, glutathione disulfide, dihydrogen monacus anka L, sodium bicarbonate (carbon dioxide), S-adenosyl-homocysteine, glutathione homocysteine transhydrogenase, glutathione-coenzyme A-glutathione transhydrogenase, S-adenosyl homocysteine, glutathione homocysteine transhydrogenase, glutathione-coenzyme A-glutathione transhydrogenase, lovastatin nonaketide synthase and stabilizing agent; samples and reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions; then the reactants are arranged under an ultraviolet/visible light analyzer to detect the increasing degree of absorbance at the 340nm position of a dominant wave so as to measure and calculate the concentration of the homocysteine.

Description

technical field [0001] The invention relates to a homocysteine ​​diagnosis / measurement reagent (box), and at the same time, the invention also relates to a method for measuring the homocysteine ​​concentration, which belongs to the technical field of medical examination and measurement. Background technique [0002] There are many methods for measuring homocysteine ​​in plasma, including high performance liquid chromatography (HPLC), amino acid analyzer assay, ion chromatography, enzyme-linked immunoassay (EIA), capillary gas chromatography-mass spectrometry, fluorescence Polarization immunoassay (FPIA), electrochemical method and enzymatic method, etc. [0003] 1. High-performance liquid chromatography (HPLC): It is a classic reference method, but it has the disadvantages of complicated operation, long test time, and large variation of test data. In clinical application, it is gradually adopted by enzyme-linked immunoassay, Fluorescence polarization analysis and enzymatic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap