Magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and preparation method thereof

A magnetic immunochromatography, quantitative detection technology, applied in the field of medical testing, can solve the problems of difficult to use in large areas, operator hazards, time-consuming, etc., to achieve the effect of convenient clinical judgment and easy operation

Inactive Publication Date: 2010-06-30
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current diagnostic techniques for total PSA+fPSA in blood mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence (CLIA), time-resolved fluorescence, colloidal gold immunochromatography and other methods, but these methods Each has its own characteristics and shortcomings: radioimmunoassay technology is mature, the results are relatively accurate, and the linear range is wide, but the operation is cumbersome and time-consuming, and the radioactive markers will cause harm to the operator and cause environmental pollution. replaced by other methods
ELISA is similar to chemiluminescence and time-resolved fluorescence method in principle, and only differs in sensitivity. At present, most of them have realized automation, large-scale, quantita

Method used

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  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and preparation method thereof
  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and preparation method thereof

Examples

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Embodiment 1

[0029] Preparation method of magnetic immunochromatography test strip and test paper box for quantitative detection of PSA and fPSA in blood

[0030] The preparation method of the present embodiment comprises the following steps:

[0031] A. Antibody preparation: commercialized total PSA+fPSA paired antibody (Hytest company, cat: 4P33) was selected, dialyzed overnight in 20 mM PBS, pH 7.24° C. for use.

[0032] B. Preparation of coating film:

[0033] Preparation of coating buffer: 0.02M phosphate buffer (PB) with pH 7.2 was used as coating buffer, sterilized by 0.22 μm microporous membrane filter and stored at 4°C for later use, valid for two weeks.

[0034] Preparation of blocking solution: 0.5% BSA, 0.02M phosphate buffered saline (PBS), 0.22 μm microporous membrane filtration and sterilization, and store at 4°C for later use, valid for one week.

[0035] Preparation of coating membrane: Dilute total PSA+fPSA antibody to 0.5mg / ml and 0.8mg / ml with coating buffer (0.02M PB...

Embodiment 2

[0062] Except for the preparation step of the magnetic particles: the molecular ratio of adding the total PSA antibody to the magnetic particles is 2:1. Other steps are with embodiment 1,

Embodiment 3

[0064] In addition to the step of preparing the magnetic particles: adding the molecular ratio of the total PSA antibody to the magnetic particles is 10:1. Other steps are the same as in Example 1.

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Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and a preparation method thereof. A coating film, a magnet particle pad combined with PSA antibodies and rabbit IgG, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A total PSA+fPSA antibody detection line and a goat anti-rabbit quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology into the quantitative detection of PSA in blood, creatively places total PSA and free PSA on the same test paper strip for simultaneous detection, and has the advantages that the detection sensitivity is improved, the accurate quantitative results of the total PSA and the free PSA can be given, the ratio between the total PSA and the free PSA is clear, and great convenience is provided for clinicians to rapidly and accurately diagnose the illness of patients.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting PSA and fPSA in blood and a preparation method thereof. Background technique [0002] Prostate-specific antigen (PSA) is a serine protease secreted by human prostate epithelial tissue cells. It is a single-chain glycoprotein molecule with a molecular weight of 30-33KDa, and the encoding gene is located at 19q13. PSA is secreted by the prostate and released into the blood of healthy men at a low concentration. In the human body, it mainly exists in the prostate tissue, semen and serum of men. In the human body, PSA mainly exists in free (fPSA) and bound state (cPSA), and the sum of the two is the total PSA. Free prostate-specific antigen (fPSA) refers to PSA in the free state, which is not combined with α1 antichymotrypsin and the like. When PSA is in the "diagnostic gray zone" (4-10ng / mL), the detection of f...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/577G01N33/531G01N33/532
Inventor 姚洪涛应希堂李强胡国茂郑金来于尚永
Owner CHEMCLIN DIAGNOSTICS CO LTD
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