Magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and preparation method thereof

A magnetic immunochromatography, quantitative detection technology, applied in the field of medical testing, can solve the problems of difficult to use in large areas, operator hazards, time-consuming, etc., to achieve the effect of convenient clinical judgment and easy operation
CN101762691AInactive Publication Date: 2010-06-30CHEMCLIN DIAGNOSTICS CO LTD
5 Cites 7 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHEMCLIN DIAGNOSTICS CO LTD
Publication Date
2010-06-30
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting PSA and fPSA in blood and a preparation method thereof. A coating film, a magnet particle pad combined with PSA antibodies and rabbit IgG, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A total PSA+fPSA antibody detection line and a goat anti-rabbit quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology into the quantitative detection of PSA in blood, creatively places total PSA and free PSA on the same test paper strip for simultaneous detection, and has the advantages that the detection sensitivity is improved, the accurate quantitative results of the total PSA and the free PSA can be given, the ratio between the total PSA and the free PSA is clear, and great convenience is provided for clinicians to rapidly and accurately diagnose the illness of patients.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting PSA and fPSA in blood and a preparation method thereof. Background technique

[0002] Prostate-specific antigen (PSA) is a serine protease secreted by human prostate epithelial tissue cells. It is a single-chain glycoprotein molecule with a molecular weight of 30-33KDa, and the encoding gene is located at 19q13. PSA is secreted by the prostate and released into the blood of healthy men at a low concentration. In the human body, it mainly exists in the prostate tissue, semen and serum of men. In the human body, PSA mainly exists in free (fPSA) and bound state (cPSA), and the sum of the two is the total PSA. Free prostate-specific antigen (fPSA) refers to PSA in the free state, which is not combined with α1 antichymotrypsin and the like. When PSA is in the "diagnostic gray zone" (4-10ng / mL), the detection of f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More