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Application of taxol and taxotere

A technology of docetaxel and paclitaxel, which is applied in the direction of medical preparations containing active ingredients, organic active ingredients, antibody medical ingredients, etc., and can solve the problem of large local irritation at the injection site, weak cellular immune response, and vaccines that cannot be frozen for storage, etc. problems, achieve the effect of reducing local irritation, improving the strength of vaccine-induced cellular and humoral immune responses, and avoiding safety trials

Inactive Publication Date: 2010-07-07
胡松华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) The existing aluminum gel adjuvant mainly promotes the humoral immune response, but has a weak effect on the cellular immune response;
[0005] 2) The local irritation reaction at the injection site caused by the existing aluminum gel adjuvant is large;
[0006] 3) Existing aluminum gel adjuvant vaccines cannot be frozen and stored, and the storage period is short
[0008] Although the anticancer effects of paclitaxel and docetaxel have been discovered for more than 40 years, the use of the above two as vaccine adjuvants has not been reported

Method used

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  • Application of taxol and taxotere
  • Application of taxol and taxotere
  • Application of taxol and taxotere

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] Example 1: The adjuvant effect of different doses of paclitaxel on mice injected with the model antigen ovalbumin (OVA)

[0031] 1. Materials and methods

[0032] 1. Laboratory animals

[0033] 60 female ICR mice were purchased from Shanghai Experimental Animal Center.

[0034] 2. Antigen

[0035] The model antigen ovalbumin (OVA) was purchased from Sigma. OVA was removed twice through an endotoxin removal column (Pierce company) to remove endotoxin, and then quantified by the BCA kit method (Pierce company).

[0036] 3. Paclitaxel (Taxol)

[0037] Purchased from Shanghai Tauto Biotech Co., Ltd. (Shanghai Tauto Biotech Co., Ltd.).

[0038] 4. Aluminum hydroxide aluminum glue

[0039] Purchased from Zhejiang Wanma Co., Ltd.

[0040] 5. Preparation of test vaccine

[0041] First dissolve paclitaxel in absolute ethanol (40mg / ml), and dissolve OVA in saline (0.25mg / ml). Add Tween-80, absolute ethanol, OVA physiological saline solution, physiological saline, paclitaxel solution or aluminu...

example 2

[0102] Example 2 The adjuvant effect of paclitaxel and docetaxel on mice injected with model antigen ovalbumin (OVA)

[0103] 1. Materials and methods

[0104] 1. Laboratory animals

[0105] Thirty female ICR mice were purchased from Shanghai Experimental Animal Center.

[0106] 2. Antigen

[0107] The model antigen ovalbumin (OVA) was purchased from Sigma. OVA was removed twice through an endotoxin removal column (Pierce company) to remove the endotoxin, and then quantified by the BCA kit method (Pierce company).

[0108] 3. Paclitaxel (Taxol) and Docetaxel (Docetaxel)

[0109] Purchased from Shanghai Tauto Biotech Co., Ltd.

[0110] 4. Preparation of test vaccine

[0111] First dissolve paclitaxel or docetaxel in absolute ethanol (40mg / ml), and dissolve OVA in normal saline (0.25mg / ml). Add Tween-80, absolute ethanol, OVA physiological saline solution, physiological saline, paclitaxel solution or docetaxel to a test tube, so that the substances contained in each 100 μl experimental vacc...

example 3

[0130] Example 3 The adjuvant effect of paclitaxel and docetaxel on the inactivation of foot-and-mouth disease virus (FMDV) in mice injected with whole virus antigen

[0131] One material and method

[0132] 1. Laboratory animals

[0133] 24 female ICR mice were purchased from Shanghai Experimental Animal Center.

[0134] 2. Antigens and adjuvants

[0135] The antigen is inactivated foot-and-mouth disease virus type O (FMDV), provided by Inner Mongolia Jinyu Biological Products Co., Ltd. Paclitaxel (Taxol) and Docetaxel (Docetaxel) were purchased from Shanghai TautoBiotech Co., Ltd..

[0136] 3. Preparation of experimental vaccine:

[0137] First dissolve paclitaxel or docetaxel in absolute ethanol (40mg / ml); dilute FMDV with saline 1:4 (volume ratio), add Tween-80, absolute ethanol, FMDV physiological saline solution to a test tube , Normal saline, paclitaxel or docetaxel solution, so that the substances contained in each 200 microliter experimental vaccine are shown in Table 3.

[0138...

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Abstract

The present invention discloses an application using taxol as vaccine adjuvant, and also discloses an application using taxotere as the vaccine adjuvant. A vaccine is a protein vaccine, a nucleic acid vaccine, a polypeptide vaccine or a whole virus vaccine. The present invention sufficiently applies a therapy that the taxol and the taxotere can activate a natural immunologic system, and the taxol and the taxotere are used as the vaccine adjuvant to be applied to the preparation of vaccines, so the strength of the immunologic reaction of cells induced by the vaccines and body liquid can be largely improved, the local simulating reaction of injection parts is reduced, the vaccines can be frozen to be preserved, and the storing time of the vaccines can be prolonged.

Description

Technical field [0001] The invention relates to the use of paclitaxel and its docetaxel as an adjuvant for preparing vaccines. Background technique [0002] Vaccines are an important means of controlling infectious diseases in humans and animals. After an ideal vaccine is used for immunization, it can not only produce sufficient immune response intensity in the immunized individual, but also produce a suitable type of immune response to exert immune protection. In the vaccine production stage, methods of increasing the amount of antigen or adding adjuvants to the vaccine are usually used to ensure the effectiveness of the vaccine. Increasing the amount of antigen increases the production cost of the vaccine and has the disadvantage of potentially increasing antigen-dependent side effects. Purified antigens can reduce the side effects of vaccines, but this increases the cost of vaccine production. Adding adjuvants to the vaccine can ensure the effectiveness of the vaccine, redu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39
CPCA61K2039/55511A61K31/7088A61K31/337A61K39/39A61P37/04A61K2300/00
Inventor 胡松华
Owner 胡松华
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