Glial cell line-Derived Neurotrophic Factor (GDNF) fusing penetrating peptides

A trophic factor, nerve cell technology, applied in hybrid peptides, nervous system diseases, recombinant DNA technology, etc., can solve HIV-TAT safety concerns, application limitations and other issues
CN101775072AActive Publication Date: 2010-07-14广州市凯诺生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
广州市凯诺生物科技有限公司
Publication Date
2010-07-14

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Abstract

The invention discloses an expression structure method for constructing Glial cell line-Derived Neurotrophic Factor (GDNF) fusing penetrating peptides, the purification of an expressed protein product and the significance thereof, which is mainly characterized in that (1) GDNF parent is a human source mature type; (2) the N-tail end of the GDNF is fused with PEP-1 penetrating peptides comprising the 21 amino acid; and the expression product of the constructed penetrating peptides GDNF fusing the gene expression plasmid has the membrane penetrating function and biological function of GDNF and can be clinically applied in the treatment of the related diseases.
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Description

technical field

[0001] The invention relates to the expression plasmid construction and expression purification of human neurotrophic factor GDNF (GDNF, the same below) fused with a penetrating peptide, and its technical field is related to life science and biomedicine technology. Background technique

[0002] Human glial cell-derived neurotrophic factor (GDNF, the same below) is a neurotrophic factor that is produced and secreted by glial cells in brain tissue and has important neurobiological functions. GDNF has a wide range of neurotrophic effects on the growth and differentiation of neurons on a variety of nerve cells in the central and peripheral areas. Dopamine neurons have the strongest effect (Lin LT et al. Science 1993.260(5111): 1130-2; Henderson et al. Science 1994, 266(5183): 1062-4).

[0003] Extensive research on the function of GDNFF at home and abroad has proved that GDNF can significantly alleviate the chromatin condensation and DNA fragmentation induced by...

Claims

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