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Detection method for measuring sugar content in environmental water sample by spectrophotometer

A spectrophotometer and detection method technology, which is applied in the preparation of test samples, analysis through chemical reaction of materials, material analysis through observation of the influence of chemical indicators, etc., can solve the problem of weak ultraviolet absorption and differential refraction. Low sensitivity of the detection method and other problems, to achieve the effect of reliable method, convenient method and easy implementation

Active Publication Date: 2010-07-14
谱尼测试集团股份有限公司
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Problems solved by technology

Due to the weak ultraviolet absorption of sugar compounds and the low sensitivity of differential refraction detection, the analysis of sugars has always been a difficult point in analytical chemistry.

Method used

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  • Detection method for measuring sugar content in environmental water sample by spectrophotometer
  • Detection method for measuring sugar content in environmental water sample by spectrophotometer
  • Detection method for measuring sugar content in environmental water sample by spectrophotometer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Precision and recovery detection of total sugar content in lake water

[0019] (1) Preparation of standard curve: Pipette 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0mL sugar standard solution (100μg / mL) into a 25mL stoppered colorimetric tube, add distilled water to 1.0mL accurately, add 1mL of 5% phenol solution, mix well (be careful to shake lightly to deflate), then add 5mL of concentrated sulfuric acid, shake well, leave it at room temperature for 40min, use a spectrophotometer at a wavelength of 490nm as a reference, and measure with a 1cm cuvette Absorbance values. Draw the standard curve with the content of glucose as the abscissa and the absorbance value as the ordinate. After determination, the equation of the standard curve is Y=0.0093X+0.0089, the linear correlation coefficient R 2 = 0.9997.

[0020] (2) Sample preparation: The lake water samples were filtered through a 0.45 μm filter membrane and placed in a stoppered Erlenmeyer flask for later use.

[00...

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Abstract

The invention relates to a detection method for measuring sugar content in environmental water sample by a spectrophotometer, and the method comprises the following steps: sucking 1mL of filtered water sample into a tube, adding phenol solution (of the concentration of 5 percent) and concentrated sulfuric acid, stirring and placing for 10 minutes, measuring a light absorption value at 490nm position after the color is displayed fully, and measuring the sugar content in the water sample according to the curve of the concentration and the light absorption value. The method for detecting the sugar content in water is rapid and effective, and the relative average deviation is less than 5.0 percent. Therefore, the method for detecting the sugar content in the environment water sample is more reliable and is easy to implement, which can meet the requirements of research and production.

Description

technical field [0001] The invention relates to a method for detecting the sugar content in an environmental water sample with glucose as the main body, in particular to a method for detecting the sugar content in the environmental water sample by spectrophotometry. Background technique [0002] Sugar is a kind of organic compound with wide distribution and relatively large content in the biological world. Almost all animals, plants, and microorganisms contain it. Sugar plays an important physiological function in the life process and is a hot spot in modern life science research. [0003] The sugar in the environmental water body is mainly glucose, and the level and change of the sugar concentration reflect the pollution status of the sugar in the water quality. Due to the weak ultraviolet absorption of carbohydrates and the low sensitivity of differential refraction detection, the analysis of carbohydrates has always been a difficult point in analytical chemistry. The p...

Claims

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Application Information

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IPC IPC(8): G01N21/79G01N1/28
Inventor 宋薇
Owner 谱尼测试集团股份有限公司
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