Detection method for measuring sugar content in environmental water sample by spectrophotometer
A spectrophotometer and detection method technology, which is applied in the preparation of test samples, analysis through chemical reaction of materials, material analysis through observation of the influence of chemical indicators, etc., can solve the problem of weak ultraviolet absorption and differential refraction. Low sensitivity of the detection method and other problems, to achieve the effect of reliable method, convenient method and easy implementation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0018] Example 1: Detection of precision and recovery rate of total sugar content in lake water
[0019] (1) Preparation of standard curve: draw 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 mL of sugar standard solution (100 μg / mL) into a 25 mL colorimetric tube with a stopper, accurately add distilled water to 1.0 mL, and add 1mL of 5% phenol solution, mix well (note gently shaking to release gas), then add 5mL of concentrated sulfuric acid, shake well, place at room temperature for 40min, use a spectrophotometer at 490nm wavelength, use reagent blank solution as reference, and measure with 1cm cuvette Absorbance values. Use glucose content as the abscissa and absorbance as the ordinate to draw a standard curve. After determination, the equation of the standard curve is Y = 0.0093X + 0.0089, and the linear correlation coefficient is R 2 = 0.9997.
[0020] (2) Sample preparation: After the lake water sample is filtered through a 0.45μm filter membrane, it is placed in a stoppered conical flas...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap