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Method for simultaneously carrying out chiral separation analysis on anisodamine, atenolol and metoprolol

A technique for separation of anisodamine and chirality, which is applied in the field of chiral separation and analysis of chiral drugs, and can solve the problem that the separation and analysis of enantiomers of metoprolol and enantiomers of atenolol cannot be realized. problem, to achieve the effect of low detection cost, high separation efficiency and high detection sensitivity

Active Publication Date: 2012-06-27
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prior art has disclosed the technology of using capillary electrophoresis electrochemiluminescence method to separate and detect substances such as amino acids, proteins, DNA and organic amines. and atenolol, using capillary electrophoresis electrochemiluminescence technology to separate and analyze metoprolol and atenolol with similar structures, but did not realize the metoprolol enantiomer and atenolol Separation and analysis of enantiomers

Method used

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  • Method for simultaneously carrying out chiral separation analysis on anisodamine, atenolol and metoprolol
  • Method for simultaneously carrying out chiral separation analysis on anisodamine, atenolol and metoprolol
  • Method for simultaneously carrying out chiral separation analysis on anisodamine, atenolol and metoprolol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Prepare NaH as follows 2 PO 4 -Na 2 HPO 4 Buffer solution:

[0043] Prepare NaH with a concentration of 200mmol / L respectively 2 PO 4 solution and 200mmol / L Na 2 HPO 4 Solution, after mixing the two solutions, dilute to a concentration of 100mmol / L with secondary water, then adjust the pH of the mixed solution to 7.0 for later use.

Embodiment 2

[0045] Follow the steps below to prepare HAc-NaAc buffer solution:

[0046] First prepare a NaAc solution with a concentration of 200mmol / L, then dilute it to 57.6mmol / L with secondary water, and then adjust the pH value of the solution to 5.3 with glacial acetic acid for later use.

Embodiment 3

[0048] The instruments used are: an uncoated fused silica capillary column with an inner diameter of 50 μm, a Pt disk working electrode with a diameter of 500 μm, an Ag / AgCl reference electrode, a Pt wire counter electrode, and MPI- A type capillary electrophoresis electrochemiluminescence detector.

[0049] With the NaH of embodiment 1 preparation 2 PO 4 -Na 2 HPO 4 The buffer solution is the background electrolyte, the scanning potential is 0-1.30V, and the cyclic voltammetry and electrochemiluminescence curves are recorded at steady state, see figure 1 a curve in and figure 2 a curve in

[0050] Add 5mmol / L ruthenium terpyridine to the background electrolyte, the scanning potential is 0-1.30V, record the cyclic voltammetry curve and the electrochemiluminescence curve when stable, see figure 1 The b curve in and figure 2 The b curve in;

[0051] Add 0.3mmol / L anisodamine, 0.3mmol / L atenolol and 0.3mmol / L metoprolol to the solution containing ruthenium terpyridyl, a...

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Abstract

The invention provides a method for simultaneously carrying out chiral separation analysis on anisodamine, atenolol and metoprolol, which comprises the following steps: putting an anisodamine sample, an atenolol sample and a metoprolol sample in a sample cell of a capillary electrophoresis electrochemistry luminescence detection hyphenated instrument and applying an injection voltage to make the samples enter a capillary of the instrument; applying a separation voltage to make the samples carry out electrophoretic separation in the capillary, wherein the capillary is filled with separation solution comprising carboxymethyl-beta-cyclodextrin and the pH value of the separation solution is between 3.0 and 6.0; and making the separated samples enter a detection cell of the instrument and applying a detection voltage to carry out detection, wherein the detection cell is filled with detection solution comprising tris (bipyridine) ruthenium and the pH value of the detection solution is between 6.0 and 9.0. Compared with the prior art, the method for carrying out chiral separation analysis, which is provided by the invention, has the advantages of less reagent use, lower detection cost, higher separation efficiency and higher detection sensitivity.

Description

technical field [0001] The invention relates to the field of chiral separation and analysis of chiral drugs, in particular to a method for simultaneous chiral separation and analysis of anisodamine, atenolol and metoprolol. Background technique [0002] Both atenolol and metoprolol belong to the class of β1 adrenoreceptor inhibitory drugs with similar structures and properties, and are widely used in the treatment of cardiovascular diseases such as hypertension, angina pectoris, arrhythmia and myocardial infarction. Anisodamine is an anticholinergic drug, which is mainly used clinically for the treatment of diseases such as smooth muscle spasm, gastrointestinal colic, and biliary tract spasm. Atenolol, metoprolol, and anisodamine are all chiral drugs, and their respective enantiomers have great differences in pharmacology, toxicology and even clinical properties. Therefore, the enantiomers of atenolol It is of great significance to separate and analyze isomers, metoprolol e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/447
Inventor 由天艳李霞于彩霞唐小风袁柏青徐春荧
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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