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Method for preparing taxifolin from fruit of princesplume ladysthumb

A technology of taxifolin and safflower seeds, which is applied in the field of preparing taxifolin, and achieves the effects of high product purity, easy decolorization and good separation effect.

Inactive Publication Date: 2010-08-18
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is no report on the extraction process of taxifolin at home and abroad. The present invention fills up the blank in this respect, and the preparation method is fast and safe.

Method used

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  • Method for preparing taxifolin from fruit of princesplume ladysthumb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 10g of safflower medicinal material, soak it with 60mL50% ethanol, place it in a crusher, crush and extract it for 15 minutes, put the extract in a centrifuge, and centrifuge it at 3000r / min for 10 minutes, repeat the operation 3 times, combine the centrifugate, and On the HPD300 macroporous resin on the centrifuge, the impurities were first eluted with 5 times the column volume of water at a flow rate of 1.5BV / h, and then eluted with 60% ethanol until no taxifolin fraction was detected by TLC, and the taxifolin fraction was collected , heated to 60°C, then added active carbon and stirred at constant temperature for decolorization for 1h, filtered, concentrated the filtrate into an extract, mixed the extract with dextran gel and dried it, then put it on a Sephadex LH-20 column in a dry method, chloroform-ethanol (6: 1) Elution, flow rate 4BV / h, thin-layer detection, collect taxifolin fraction, concentrate under reduced pressure to recover the solvent, and obtain the...

Embodiment 2

[0024] Take 10g of safflower medicinal material, soak it with 100mL45% ethanol, place it in a crusher and extract it for 20 minutes, put the extract in a centrifuge, and centrifuge it at 3500r / min for 15 minutes, repeat the operation twice, combine the centrifugate, and On the NKA-9 macroporous resin on the centrifuge, the impurities were first eluted with 4 times the column volume of water at a flow rate of 2 BV / h, and then eluted with 50% ethanol until no taxifolin fraction was detected by TLC, and the taxifolin flow was collected Separate, heat to 60°C, add active carbon and stir at constant temperature for decolorization for 1h, filter, concentrate the filtrate into an extract, mix the extract with dextran gel and dry it, then put it on a Sephadex LH-20 column in a dry method, chloroform-ethanol (9 : 1) elution, flow rate 4BV / h thin-layer detection, collect taxifolin fraction, concentrate under reduced pressure to recover the solvent, to obtain crude product, benzene-ethano...

Embodiment 3

[0026] Take 20g of Safflower medicinal material, soak it with 240mL A0% ethanol, place it in a crusher and extract it for 10 minutes, put the extract in a centrifuge, and centrifuge it at 4000r / min for 12 minutes, repeat the operation 3 times, combine the centrifugate, and On the HPD600 macroporous resin on the centrifuge, the impurities were first eluted with 5 times the column volume of water at a flow rate of 3 BV / h, and then eluted with 75% ethanol until no taxifolin fraction was detected by TLC, and the taxifolin fraction was collected. Heat to 60°C, add activated carbon and stir at constant temperature for decolorization for 1 hour, filter, concentrate the filtrate into an extract, mix the extract with dextran gel and dry it, then put it on a Sephadex LH-20 column by dry method, chloroform-ethanol (5:1 ) elution, flow rate 1.5BV / h, thin-layer detection, collect taxifolin fraction, concentrate under reduced pressure and recover the solvent to obtain a crude product, benzen...

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Abstract

The invention belongs to the traditional Chinese medicine field and provides a method for preparing taxifolin from fruit of princesplume ladysthumb. The method concretely includes that: dilute alcohol crushing extraction is adopted, extract is subject to centrifugation and then is put on chromatographic column, firstly water is used for washing impurities away, then ethanol is used for eluting, eluent is destained by active carbon, destaining solution is put on chromatographic column again, chloroform-ethanol is used for eluting, reduced pressure concentration, recrystallization and drying are carried out, and clear crystal is obtained. The invention has the characteristics that crushing extraction is adopted, speed is fast, the process is safe, and a lot of time can be saved.

Description

Technical field: [0001] The invention relates to the technical field of traditional Chinese medicine, and relates to a method for preparing taxifolin from Saffron chinensis. Background technique: [0002] Taxifolin, also known as luteolin and toxin, is a dihydroflavonoid compound with a chemical name of 5,7,3',4'-tetrahydroxydihydroflavonol. [0003] Molecular formula: C 15 h 12 o 7 ;Molecular weight: 304.25; [0004] [0005] Molecular Structure: [0006] Physical and chemical properties: It is a colorless needle-like crystal, with a melting point of 208-210°C. Soluble in ethanol, acetic acid, boiling water, slightly soluble in cold water, almost insoluble in benzene. [0007] The traditional Chinese medicine Shuihonghuazi is the dried and mature fruit of Polygonum orentale L., a plant of the Polygonaceae family. It mainly contains flavonoids. No need, epigastric pain. [0008] Taxifolin can affect lipid metabolism by regulating the activity of enzymes; it can in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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