VSIG4 (V-set and Ig domain containing 4) and IGRP (glucose-6-phosphatase catalytic subunit-related protein) dual-gene coexpression recombinant adenovirus as well as preparation method and application thereof

Abstract

The invention discloses a VSIG4 (V-set and Ig domain containing 4) and IGRP (glucose-6-phosphatase catalytic subunit-related protein) dual-gene coexpression recombinant adenovirus as well as a preparation method and an application thereof. The recombinant adenovirus genome contains a VSIG4 and IGRP dual-gene expression cassette which sequentially comprises a CMV (cytomegalovirus) promoter, a VSIG4 full length coding gene, a terminator, an internal ribosome entry site (IRES), an IGRP full length coding gene and a terminator from the upstream to the downstream. The preparation method of the recombinant adenovirus is simple. Dendritic cells of the recombinant adenovirus, which are transfected in vitro, are fed back in vivo, which can reduce the multiplication capacity of lymphocytes of NOD (Nonobese Diabetic) mice with diabete liability and the secretion capacity of cell factors, moreover, the onset time of the diabetes of the NOD mice is delayed, and the morbidity is reduced. The recombinant adenovirus is proved to be capable of effectively inducing the tolerance of specific T-cells and effectively inhibiting the breakage of pancreas islet beta cells and can be used for preparing dendritic cell vaccines for resisting type-1 diabetes.

Description

technical field [0001] The invention relates to a recombinant adenovirus, in particular to a VSIG4 and IGRP double gene co-expression recombinant adenovirus, and also relates to a preparation method and application of the recombinant adenovirus. Background technique [0002] Type 1 diabetes is an autoimmune disease caused by the body's immune system attacking the beta cells of the pancreas, disrupting their ability to secrete insulin. At present, it mainly relies on insulin and islet transplantation for treatment. Although it can reduce the mortality of patients, there are a lot of side effects. With the deepening of research, experiments have found that T cells play an important role in the pathogenesis of type 1 diabetes mediated by the immune system, and specifically inducing T cell-mediated immune tolerance is a promising strategy for type 1 diabetes. treatment strategy. [0003] In the T cell-mediated immune response, the activation of T cells not only requires comple...

AI Technical Summary

Problems solved by technology

[0005] There are two main methods of gene combination therapy: one is to simultaneously transfect target cells with a variety of recombinant expression vectors carrying different genes. The construction of recombinant expression vectors is cumbersome, time-consuming, and has many influencing factors. In addition, due to the randomness of the transfection process, the transfected cells have the problem of uneven gene copies, which is not conducive to the expression of the target gene and subsequent treatment. It makes the experimental results difficult to evaluate and analyze; the above shortcomings limit the further application of this method

Images