Method for cultivating lactobacillus and producing live lactobacillus feed by utilizing scum
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A technology of lactic acid bacteria and sugar residue, applied in the field of bioengineering, to achieve the effect of increasing the number of viable bacteria and reducing costs
Inactive Publication Date: 2010-09-08
JIANGNAN UNIV
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[0004] However, sugar slag still contains certain nutritional components. According to our analysis and determination, reducing sugar accounts for about 20%, crude protein accounts for about 30%, and is rich in inorganic salts. However, compared with conventional lactic acid bacteria culture medium components, A lot of work still needs to be done to cultivate lactic acid bacteria in the medium with sugar residue as the main component
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Embodiment 1
[0032] Embodiment 1. take starch sugar residue as the main raw material liquid batch culture of lactic acid bacteria
[0033] Bacteria: Lactobacillus rhamnosus.
[0034] (1) Preparation of seed solution
[0035] Seed medium: MRS medium
[0036] Strain preservation medium: MRS medium
[0037] Strain preservation method: 20% glycerol is stored at -40°C
[0038] Seed solution preparation method: put the preserved strains in an incubator at 37±5°C for static activation for 30 minutes, inoculate them into the seed medium at a ratio of 5% to 15%, and culture them statically at 37±5°C for 10-12 hours.
[0039] (2) Liquid culture
[0040] Liquid fermentation medium (g / L): sugar residue 100-200, peptone 7-15, MgSO 4 0.2~0.6, MnSO 4 0.03~0.1, after adjusting the pH, add appropriate amount of CaCO 3 To maintain a stable pH during fermentation.
[0041] Liquid fermentation conditions: inoculum size: 10%, culture temperature after inoculation: 37±5°C, static culture.
[0042] Fe...
Embodiment 2
[0052] Embodiment 2. take starch sugar residue as raw material for solid-state culture of lactic acid bacteria
[0053] Bacteria: Lactobacillus rhamnosus
[0054] (1) Preparation of seed liquid: with embodiment 1.
[0055] (2) Solid state culture
[0056] Solid-state fermentation medium: mainly sugar residue, with a small amount of bran added.
[0057] Solid-state fermentation conditions: inoculum size: 20%, cultivation temperature after inoculation: 37±5°C, humidity 40%-80%, fermentation time: 24h-36h.
[0058] Solid-state culture method: insert the seed solution into the fermentation medium with 20% inoculum amount, measure the OD value of the seed solution before inoculation, stir the material evenly and place it in an incubator at 37±5°C for 24h-36h to measure the number of viable bacteria .
[0059] (3) Detection of viable bacteria: the detection method of viable bacteria adopts the plate colony counting method.
[0060] The effect of raw material composition on the ...
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Abstract
The invention relates to a method for cultivating lactobacilli and producing live lactobacillus feed by utilizing scum, which belongs to the field of biological engineering. The method comprises the following steps: using the scum which is a byproduct of a cornstarch sugar manufacturing industry as a main raw material, using feeding lactobacilli as starting bacteria, and carrying out solid state cultivation of the lactobacilli to prepare feed containing live lactobacilli; and cultivating the lactobacilli through liquid state fermentation to prepare a live lactobacillus preparation. In the invention, the scum is used as the main raw material, the optimization of culture medium components and the optimization of cultural conditions are researched in details to obtain optimization process parameters, the feeding lactobacilli are cultivated at high density by adopting the modes of solid state batching, liquid state batching or liquid state supplementation, high-grade feed rich in the live lactobacilli is cultivated through solid state or liquid state fermentation, the content of the live lactobacilli through liquid state cultivation can reach more than 109cfu / mL, and the content of the live lactobacilli through solid state cultivation can reach more than 108cfu / g. In the invention, the scum is used as the main raw material for cultivating the feeding lactobacilli, so that waste is changed into resources, and the cost for producing the lactobacilli is lowered.
Description
technical field [0001] The invention provides a method for cultivating feed rich in lactic acid bacteria with high density and high production intensity by using sugar residue (hereinafter referred to as sugar residue), a by-product of starch sugar production, as a main raw material. It belongs to the field of bioengineering. The produced lactic acid bacteria preparation or The feed of lactic acid bacteria can be applied to the feed industry. Background technique [0002] The nutritional requirements for the growth of lactic acid bacteria are relatively high. In addition to sufficient carbon sources, nitrogen sources, and inorganic salts, almost every type of lactic acid bacteria has its own special growth factor requirements, such as vitamins, small molecule amino acids, and nucleic acid derivatives. etc. Therefore, the raw materials of the medium (whey powder, various milk powders, MRS medium, etc.) used for cultivating lactic acid bacteria preparations at home and abroad ...
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