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Method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization

A technology for fermentation liquid and floc, which is applied in the field of separation and extraction of lactic acid in fermentation liquid, can solve the problems of safety, lack of comprehensive utilization, waste of resources and the environment, etc., and achieves the effects of reducing cost, good solid-liquid separation effect, and improving yield.

Inactive Publication Date: 2012-11-28
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the flocculation process of the above invention, the flocculant is one of the main costs, but the sediment obtained by flocculation, including a large amount of protein, bacteria, residual sugar, etc., has not been comprehensively utilized, resulting in waste of resources and potential environmental security issues

Method used

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  • Method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization
  • Method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 50ml of fermented liquor of industrial liquor distillers grains, adjust the pH to 5, heat to 30-60°C, add chitosan according to Table 1, stir at normal speed for about 5min to appear obvious flocs, let it settle for 30min, pour out the supernatant After diluting 10 times, measure its absorbance value and lactic acid concentration at 650nm.

[0019] Table 1 Comparison of effects before and after adding chitosan flocculation

[0020]

[0021] *1 Flocculation rate=[(absorbance value before flocculation-absorbance value after flocculation) / absorbance value before flocculation]×100%

[0022] *2 Lactic acid loss rate = [(lactic acid concentration before flocculation - lactic acid concentration after flocculation) / lactic acid concentration before flocculation] × 100%

[0023] Carry out fermentation test according to table 2: industrial liquor distiller's grains 5g, add water according to solid-liquid ratio 1: 10, add 1g chitosan floc, put into 100ml Erlenmeyer flask...

Embodiment 2

[0027] Take 50ml of fermented liquor of industrial liquor distillers grains, adjust the pH value to 8, heat to 30-60°C, add CPAM according to Table 3, stir at normal speed for about 5min to appear obvious flocs, let it settle for 30min, pour out the supernatant, dilute Measure its absorbance value and lactic acid concentration at 650nm after 10 times.

[0028] Table 3 Comparison of effects before and after adding CPAM flocculation

[0029]

[0030] *1 Flocculation rate=[(absorbance value before flocculation-absorbance value after flocculation) / absorbance value before flocculation]×100%

[0031] *2 Lactic acid loss rate = [(lactic acid concentration before flocculation - lactic acid concentration after flocculation) / lactic acid concentration before flocculation] × 100%

[0032] Carry out the fermentation test according to Table 4: 10g of industrial liquor distillers grains, add water according to the solid-to-liquid ratio of 1:5, add 2g of CPAM flocs, put into a 100ml Erl...

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Abstract

The invention provides a method for separating lactic acid from fermentation liquor by flocculation and carrying out flocculate comprehensive utilization, belonging to the technical filed of industrial microbiology fermentation engineering. In the method, a pre-treatment is carried out before fermentation liquor separation by flocculation, and then obtained flocculate is returned to a fermentation tank and fermented with organic waste to produce lactic acid. The method comprises the following processing steps: 1) adding ammonia water or sodium hydroxide in the fermentation liquor for alkalization and heating the fermentation liquor; 2) adding a flocculant in the fermentation liquor, stirring until visible flocculate appears, and standing for obvious layering; and 3) separating solid from the liquor, and allowing the liquid phase to enter subsequent membrane separation lactic acid purification workstage and the solid phase to return to the fermentation tank to ferment with organic waste so as to improve lactic acid yield. The invention has the advantages of simple operation, high flocculation rate, easy separation of solid from liquor, low cost and little loss to target product of lactic acid, realizes flocculate comprehensive utilization, and reduces cost of flocculation pre-treatment of fermentation liquor.

Description

Technical field: [0001] The invention belongs to the technical field of industrial microbial fermentation engineering, and in particular relates to a method for separating and extracting lactic acid in fermentation liquid. Background technique: [0002] Lactic acid, also known as propionic acid, scientific name 2-hydroxypropionic acid, is an important organic carboxylic acid. Lactic acid has been widely used in food, chemical, pharmaceutical, cosmetic and other industries as sour agent, preservative, emulsifier, bactericide and pharmaceutical intermediate. In recent years, as an important raw material for the synthesis of biodegradable thermoplastic material polylactic acid, the production of lactic acid has been paid more and more attention. The production of lactic acid is usually carried out by fermentation with the aid of microorganisms such as bacteria, yeasts and fungi, the fermentation substrate consists of carbohydrates and suitable mineral and protein nutrients. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C59/08C07C51/42C12P7/56C12R1/245
Inventor 汪群慧王利娟刘瑛颖
Owner UNIV OF SCI & TECH BEIJING
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