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Flammulin hyphostroma with high anti-cancer activity

A technology of anticancer activity and mushroom fungus, which is applied in the field of mycelium to achieve the effect of enhancing anticancer activity

Inactive Publication Date: 2010-09-08
YANTAI DAYANG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fungal culture technology shows that trace elements can affect the yield of extracellular polysaccharides in mycelia, but the effects on the composition and activity of polysaccharides have not been reported.

Method used

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  • Flammulin hyphostroma with high anti-cancer activity
  • Flammulin hyphostroma with high anti-cancer activity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Test tube bacteria

[0044] Provided by the General Microbiology Center of China Committee for Culture Collection of Microorganisms, the strain number and name are as follows: AS 5.474 Flammulina velutipes Flammulina velutipes.

[0045] 2. Cultivation of slant strains

[0046] Medium: Potato 200g / L (peeled, cut into small pieces, boiled for half an hour, filtered, take the filtrate), glucose 2.0g / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 1g / L, magnesium sulfate (MgSO 4 ) 1g / L, yeast powder 5g / L, agar 20g / L, and the pH value is natural. Sterilized by 0.1Mpa, 121°C, 30min.

[0047] Inoculate the bacteria in the test tube onto the slant medium and place it in a constant temperature incubator for cultivation at 25±1°C until the slant is covered with thick, dense, white and fine powder mycelia.

[0048] 3. Primary shake flask strain culture

[0049] The medium formula is: soybean cake powder 15g / L, glucose 10g / L, sucrose 20g / L, potassium dihydrogen phosphate (K...

Embodiment 2

[0070] 1. Test tube bacteria

[0071] Provided by the General Microbiology Center of China Committee for Culture Collection of Microorganisms, the strain number and name are as follows: AS 5.474 Flammulina velutipes Flammulina velutipes.

[0072] 2. Cultivation of slant strains

[0073] Medium: Potato 200g / L (peeled, cut into small pieces, boiled for half an hour, filtered, take the filtrate), glucose 2.0g / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 1g / L, magnesium sulfate (MgSO 4 ) 1g / L, yeast powder 5g / L, agar 20g / L, and the pH value is natural. Sterilized by 0.1Mpa, 121°C, 30min.

[0074] Inoculate the bacteria in the test tube onto the slant medium and place it in a constant temperature incubator for cultivation at 25±1°C until the slant is covered with thick, dense, white and fine powder mycelia.

[0075] 3. Primary shake flask strain culture

[0076] The medium formula is: soybean cake powder 15g / L, glucose 10g / L, sucrose 20g / L, potassium dihydrogen phosphate (K...

Embodiment 3

[0097] 1. Test tube bacteria

[0098] Provided by the General Microbiology Center of China Committee for Culture Collection of Microorganisms, the strain number and name are as follows: AS 5.474 Flammulina velutipes Flammulina velutipes.

[0099] 2. Cultivation of slant strains

[0100] Medium: Potato 200g / L (peeled, cut into small pieces, boiled for half an hour, filtered, take the filtrate), glucose 2.0g / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 1g / L, magnesium sulfate (MgSO 4 ) 1g / L, yeast powder 5g / L, agar 20g / L, and the pH value is natural. Sterilized by 0.1Mpa, 121°C, 30min.

[0101] Inoculate the bacteria in the test tube onto the slant medium and place it in a constant temperature incubator for cultivation at 25±1°C until the slant is covered with thick, dense, white and fine powder mycelia.

[0102] 3. Primary shake flask strain culture

[0103] The medium formula is: soybean cake powder 15g / L, glucose 10g / L, sucrose 20g / L, potassium dihydrogen phosphate (K...

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Abstract

The invention relates to a flammulin hyphostroma with high anticancer activity and flammulin polysaccharides FVP-1, FVP-2 and FVP-3 in specific proportions. The flammulin hyphostroma is obtained by mainly adopting a method of adding a specific trace element association into a culture medium and then performing culture and fermentation under specific process parameters, wherein the ratio of the FVP-1 to the FVP-2 to the FVP-3 of the flammulin hyphostroma is close to 1:2:2. An in vitro cell culture experiment and an animal experiment show that the anticancer activity of the flammulin hyphostroma is obviously higher than that of the flammulin hyphostroma obtained by a common fermentation mode, and the flammulin hyphostroma can be used for preparing anticancer medicaments and health products.

Description

technical field [0001] The invention relates to a mycelium with improved anticancer activity, in particular to a mushroom mycelium with a specific proportion of pleurotus polysaccharide components. Background technique [0002] Pleurotus velutipes (Flammulina velutipes) belongs to Tricholomaceae, Chrysanthemum genus. In recent years, the anticancer activity of FVP (Flammulina velutipes Polysaccharide) contained in its mycelium has attracted increasing attention. Tumor histomorphological and histochemical observations found that FVP can inhibit tumor growth, increase the degree of tumor necrosis, reduce glycogen, and decrease the activity of acidic non-specific esterase in tumor tissue. [0003] FVP is a general term for a series of Pleurotus polysaccharide molecules, which are divided into FVP-1 according to the length of the carbon chain contained (relative molecular weight 1.48×10 4 ), FVP-2 (relative molecular weight 2.73×10 4 ), FVP-3 (relative molecular weight 5.4×10...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61P35/00A01G1/04C12R1/645
Inventor 杨修镇
Owner YANTAI DAYANG PHARMA GRP