Method for breading shrimp postlarvae by biomanipulation

A breeding method and technology of shrimp seedlings, applied in the field of aquatic seedlings, to achieve the effects of enhancing disease resistance, good investment returns, and reducing ammonia nitrogen and nitrite nitrogen

Inactive Publication Date: 2010-09-15
李活
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most livestock breeds and experimental animals have established specific pathogen-free populations, and catfish, ca

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0012] Example:

[0013] 1. Treatment of seedling water: The seedling water should be precipitated in the dark for 24 hours and filtered with secondary sand. The primary sand filter is filtered through a coarse sand layer with a thickness of 50 cm and a particle size of 0.2 to 0.3 cm; the volume of the secondary sand filter is 12 cubic meters, and the basic space and sieve plate of about 10 cm at the bottom of the sand filter must be used as Support; a layer of pebbles with a diameter of 4 cm are laid on the sieve plate; coarse sand with a thickness of 50 cm and a particle size of 0.1 to 0.2 cm is laid on the gravel; . After sand filtration, sodium hypochlorite needs to be added to the seawater to make the available chlorine in the water reach 12.5×10 -6 , sterilized for 9 hours, and then added 10×10 -6 Neutralize with sodium thiosulfate, remove excess chlorine, and use it after full blasting for 12 hours.

[0014] 2. Selection, cultivation and breeding of broodstock: choo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for breading shrimp postlarvae by biomanipulation. The method comprises the following steps of: selecting the parent shrimp without virus WSSV and TSV, and feeding the parent shrimp with bait without virus WSSV and TSV, wherein the feeding amount per day is 4 to 6 percent of the weight and the parent shrimp is fed for four times; breading the parent shrimp under the conditions that the water temperature is between 26 and 30 DEG C, the salinity is 2.8 to 3.2 percent and the illumination intensity is 100 to 1,000 lx to make the parent shrimp reach sexual maturity; after mating, putting the female shrimp into a spawning pond to make female shrimp lay eggs, and washing and sterilizing the collected fertilized ovum; incubating the fertilized ovum into the nauplius under the conditions that the water temperature is between 28 and 31 DEG C, the salinity is 2 to 3 percent, the pH value is 7.8 to 8.6, dissolved oxygen is 6 to 10mg/l after 13 to 18 hours, putting the nauplius into a nursing pond which is used for culturing seedlings and treated by the water, wherein the density of the nauplius is 200-300 thousand/m<3>; in the late-mid period of the nauplius growing, introducing chaetoceros muelleri without virus according to the concentration of 5*104cell/ml, and when breading nauplius the into the zoea, introducing bacillus subtilis according to the concentration of 1.25*104cfu/ml; and after the shrimp is bred to 0.8 to 1cm, obtaining the seedlings. The whole breading process is subjected to regular PCR detection, and no specific pathogen WSSV and TSV is ensured in the whole breading process. The method has the advantages of no specific virus, rapid growth, high disease resistance, high stress resistance, short breeding period, large and uniform growing standard, high yield, good investment benefit and the like.

Description

technical field [0001] The invention relates to a biologically regulated method for cultivating shrimp seedlings without specific pathogens, and belongs to the technical field of aquatic seedling cultivation. Background technique [0002] Specific pathogen-free animals are animals in which no specific viruses, microorganisms or parasites exist. Specific pathogen-free refers to the specific current status of a specific pathogen, not the resistance of the pathogen or the status of the future pathogen. Specific pathogen-free is a concept of pathogen control, not a genotype or phenotype of animal genetics, which is fundamentally different from genetic concepts such as animal species, breeds, varieties or strains. Importation of specific pathogen-free animals will not result in the introduction of specific pathogens. At present, most livestock species and experimental animals have established specific pathogen-free populations, and there are also reports of specific pathogen-fr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 李活黄翔鹄李长玲宋文东陆家昌罗杰
Owner 李活
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap