Method for separating and purifying blue algae polysaccharide

Abstract

The invention discloses a method for separating and purifying blue algae polysaccharide, which belongs to the technical field of biochemical separation. The method comprises the following steps of: preparing a crude extract of the blue algae polysaccharide; treating the crude extract of the blue algae polysaccharide by using a flocculating agent; performing vacuum concentration on the crude extract treated by using the flocculating agent; depositing the blue algae polysaccharide by using ethanol; and eluting a deposit and then performing vacuum drying on the deposit to obtain the blue algae polysaccharide with certain purity. The method relates uses the flocculating agent to flocculate impurities, but does not adopt the common isoelectric point, sevage method and the like to remove the impurities such as protein and the like. The method simplifies steps of the purification process, has simple operation, reduces the used amount of chemical reagents used in the preparation process, and saves resources, and the purity of the obtained polysaccharide can reach as high as 91.18 percent. Fresh or dried algae powder, even field Taihu Lake water bloom blue algae is used as a raw material, so a technical method for realizing scale utilization of algae resources is provided.

Description

technical field [0001] The invention provides a simple and fast method for extracting and separating blue-green algae polysaccharides from blue-green algae. It belongs to the technical field of biochemical separation. Background technique [0002] Studies by domestic and foreign personnel have shown that algae have various functions such as anti-fatigue, anti-radiation, anti-virus, anti-tumor, anti-allergy, and enhancing immunity, which determines that algae and its active ingredients have a wide range of functions in the research and development of functional foods. Application prospects. The biological activities of seaweed polysaccharides are mainly manifested in anticoagulant, antiviral, immune regulation and antitumor effects. The spatial structure of polysaccharides and the molecular weight of polysaccharides are related. [0003] There have been many research reports on polysaccharides and their biological activities of red algae, brown algae and green algae, but l...

AI Technical Summary

Problems solved by technology

[0004] The development and utilization of economical microalgae in my country is at the primary processing stage, and the extraction and separation and purification of seaweed polysaccharides mostly stay in the laboratory research stage. The reported patented technology of algae polysaccharides, such as Chinese patents CN1297948A, CN1397567A, CN101445565A, etc. Isoelectric point method, sevage method, trichloroacetic acid method, etc. to remove protein, and ion exchange column to purify polysaccharide, although the purity of polysaccharide is improved to a certain extent, but the production cost increases and the process is cumbersome; CN1392360A, CN101037483A use active amino and hydroxyl The cationic bioflocculant chitosan or modified starch, or the anionic flocculant that ionizes sulfonate groups when dissolved in water to extract seaweed polysaccharides, the addition amount is 50ppm ~ 500ppm, and the price is relatively inorganic macromolecule or polyacrylamide flocculation dose higher