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Method for rapidly dissolving chitosan and preparing chitosan with high-concentration substrate enzymatic method

A technology of enzymatic preparation and chitosan, applied in fermentation and other directions, can solve the problems of slow dissolution rate of chitosan, high production cost, low substrate concentration, etc., and achieve shortened dissolution time, high product quality, and substrate concentration. high effect

Inactive Publication Date: 2010-09-29
JIANGSU SHUANGLIN MARINE BIOLOGICAL PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of fast dissolving chitosan that can effectively overcome the bottleneck problem of slow dissolving speed of chitosan, low substrate concentration, high energy consumption and high production cost under the prior art conditions, and enzyme with high substrate concentration Method for preparing chitosan oligosaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Add 250g of chitosan and 5L of water into the high-speed shear disperser, run at a speed of 1500rpm or more for 1min, then add 100mL of glacial acetic acid, and after running for 5min, add 2500U of chitosan hydrolase, and control the temperature between 43°C and 48°C , when the viscosity drops below 1000cp and pH > 4.3, run at 3000rpm, add chitosan powder continuously, add glacial acetic acid to dissolve, and add chitosan hydrolase according to the amount of chitosan hydrolase mentioned above The amount of acetic acid added is 2% of the volume of the solution to be added with acetic acid until the concentration of the final chitosan solution reaches 10%, and the pH is 4.6. Add 10,000 U of chitosan hydrolase to the solution, and stir for 24 hours at a temperature of 42° C. to 48° C. Use 5M NaOH to adjust the pH of the system to 7.0, let it stand for 60 minutes to form flocs, and centrifuge at 3000rpm to remove water-insoluble chitosan and other impurities. The supernata...

Embodiment 2

[0032] Add 250g of chitosan and 5L of water into the high-speed shear disperser, run at a speed of 1500rpm or more for 1min, then add 100mL of glacial acetic acid, and after running for 5min, add 12,500U of chitosan hydrolase, and control the temperature between 45°C and 50°C During this period, the viscosity drops below 1000cp, and the operation is performed at 3000rpm. The chitosan powder is added continuously, the glacial acetic acid is added to dissolve, and the chitosan hydrolase is added until the concentration of the final chitosan solution reaches 15%, and the pH is 4.6. Add 40,000 U of chitosan hydrolase to the solution at a temperature of 45° C. to 50° C., and stir for 5 hours. All the other processing steps are the same as in Example 1.

Embodiment 3

[0034] According to the steps of Example 1, continue to add chitosan to make the final substrate concentration reach 20%, the consumption of chitosan hydrolase in the enzymolysis reaction is 20,000U, pH4.5, temperature 43 ℃~48 ℃, reaction The time is 24h. Finally, light yellow chitosan oligosaccharide powder was obtained.

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Abstract

The invention discloses a method for rapidly dissolving chitosan and preparing chitosan with high-concentration substrate enzymatic method; a high-speed shearing dispersion machine is adopted to rapidly dissolve and disperse the chitosan to form high-concentration substrate solution, and the adding part is utilized to degrade hydrolytic enzyme of the chitosan to lead the viscosity of the solution to be diluted, and the concentration of the chitosan substrate in a reaction system is improved to 10-20 percent. After enzyme reaction is carried out, chitosan hydrolytic enzyme protein and chitosan macromolecules in product solution are removed by an ultrafiltration method, permeate is concentrated for desalinating through nanofiltration, and the solid content of the chitosan in the product can reach 20-35 percent through vacuum concentration process; the method can effectively solve the problems that the dissolution time of the chitosan is long, the produced solution concentration is low, and energy consumption is high owning to low substrate concentration, the energy consumption level of subsequent processing such as separation purification, concentration and drying processes is greatly reduced, and the production cost is lowered.

Description

technical field [0001] The invention relates to the industrialized production technology of chitosan oligosaccharide, in particular to a method for rapidly dissolving chitosan by a high-speed shearing disperser and enzymatically producing chitosan oligosaccharide under the condition of high substrate concentration. Background technique [0002] Chitooligosaccharides are oligosaccharides with a degree of polymerization of 2 to 20 generated by hydrolysis of chitosan, and are oligomers composed of glucosamine or a small amount of N-acetylglucosamine. Chitosan oligosaccharide not only maintains some functional properties of chitosan macromolecules, but also has many unique physiological activities and functional properties that chitosan does not have. It is extremely important in the fields of food, medicine, cosmetics and agriculture. It has been widely used and has become an emerging functional oligosaccharide in the world. What is particularly striking is that it has high ac...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P19/00
Inventor 夏文水白云鹏姜启兴梁双林彭秀兰
Owner JIANGSU SHUANGLIN MARINE BIOLOGICAL PHARM CO LTD
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