Normalized cDNA library of ocean medaka specific tissue and preparation method thereof

A medaka and specific technology, applied in the field of cDNA library, can solve the problems of incomplete genetic information, high proportion of copies, no product seen, etc., and achieve the effect of convenient analysis and research
CN101851786AInactive Publication Date: 2010-10-06香港城市大学深圳研究院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
香港城市大学深圳研究院
Publication Date
2010-10-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a normalized cDNA library of ocean medaka specific tissues and a preparation method thereof, belonging to the field of cDNA libraries. The library is prepared from one or more specific tissues of ocean medaka. The preparation method of the normalized cDNA library comprises the following steps: extracting total RNA of the specific tissues of the ocean medaka, synthesizing cDNA polynucleotide products, re-hybridizing after cDNA denaturation, selectively degrading double-stranded components, amplifying normalized cDNA components through PCR and inserting amplified products in carriers. The normalized cDNA library of ocean medaka specific tissues lays a foundation for greatly finding and researching the expressing genes of the ocean medaka, and is of a great significance to the deep research on the expressing markers of the ocean medaka specific tissues.
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Description

technical field

[0001] The invention relates to the field of cDNA libraries, in particular to a uniform cDNA library of marine medaka fish-specific tissues and a preparation method thereof. Background technique

[0002] Marine medaka (Oryzias Melastigma) is commonly found in the waters of China, North Korea, Japan, and India. Adults are about 3.5-4 cm long and have a life cycle of about 2-3 months. The genome is small, only half of the zebrafish genome , about 800Mb, mild in character, high in spawning rate, easy to observe, low in seawater consumption, more tolerant to seawater environmental pressure, and the liver and reproductive tissues are more sensitive to environmental pressure changes caused by hypoxia, so it is very suitable as a Model organisms are used in scientific research, especially for experimental research in marine environmental quality assessment and marine ecotoxicology. However, the genetic information of marine medaka fish is still incomplete. Searchin...

Claims

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