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Grifola frondosa extract and composition containing the same for promoting the production of hyaluronic acid (hyaluronan)

A technology of hyaluronic acid and extracts, applied in drug combination, medical raw materials derived from fungi, skin diseases, etc., can solve problems such as ignorance, and achieve the effect of promoting production, improving damage, abnormality and disease

Inactive Publication Date: 2010-10-06
HEIMAT +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the regulation of the expression of extracellular matrix (ECM)-associated molecules by pure ethanol extracts of dried maitake fruiting bodies has been unknown until now.

Method used

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  • Grifola frondosa extract and composition containing the same for promoting the production of hyaluronic acid (hyaluronan)
  • Grifola frondosa extract and composition containing the same for promoting the production of hyaluronic acid (hyaluronan)
  • Grifola frondosa extract and composition containing the same for promoting the production of hyaluronic acid (hyaluronan)

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Experimental program
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Effect test

Embodiment 1

[0070] Hereinafter, the present invention will be described with examples and the like, but the present invention is not limited thereto.

[0071] 1. Manufacture of Maitake Extract

[0072] Add 4000 g of pure ethanol (moisture content: 99.5% by volume or more) to 1000 g of sterilized dry powder of maitake mushroom (moisture content: 6% by weight, maitake mushroom manufactured by Hokuto Co., Ltd.), and heat at 20°C The extraction was carried out under stirring for 18 hours. The residue was removed by centrifugation, and the obtained supernatant was filtered through filter paper. Ethanol was evaporated from the obtained filtrate to obtain a pure ethanol extract of maitake mushroom (yield: 43.2 g, including 37.2 g of solid content and 6.0 g of ethanol).

[0073] 2. Culture and processing method of human fibroblasts

[0074] Normal human fibroblast cells NB1RGB (RCB0222) were purchased from the Biological Resource Center of the Institute of Physical Chemistry and Chemistry (Ger...

Embodiment 2

[0104] experimental method

[0105] 1. Preparation of components from the Maitake mushroom extract prepared in Example 1

[0106] The maitake extract (8.88 g) prepared in Example 1 was dissolved in 15 ml Dissolve in chloroform-methanol (19:1), add to normal phase silica gel, change the mobile phase in turn (chloroform-methanol (19:1, use 1000ml)→chloroform-methanol (9:1, use 800ml)→chloroform- Methanol (4:1, 800ml used)→methanol (1000ml used)) was separated, and about 60ml each was collected. The first fraction obtained was designated as Fr1, and a total of 60 fractions were isolated. Next, a part of the separated total fraction (Fr1-60) was taken out, and a developing solvent (chloroform-methanol (9 : 1)) unfold (expand distance: 8cm). After irradiating the TLC plate with UV (254 nm), spraying a 10% sulfuric acid reagent, and then heating, the separated components were detected. As a result, the following five components (M1 to M5) composed of the same component group we...

Embodiment 3

[0110] experimental method

[0111] 1. Preparation of components from the Maitake extract component 5 (M5) prepared in Example 2

[0112] The maitake extract fraction 5 (M5) prepared in Example 2 was purified by column chromatography using normal-phase silica gel (BW-300, manufactured by Fuji Silicon Chemical Co., Ltd.; using a glass hollow column with an inner diameter of 40 mm × 300 mm). (1.2g) was dissolved in 20ml of chloroform-methanol-water (4:1:0.1), added to normal phase silica gel, and the mobile phase was changed sequentially (chloroform-methanol-water (4:1:0.1, using 1700ml)→ Methanol (using 300ml)) was separated, and about 50ml each was collected. The first fraction obtained was designated as Fr1, and a total of 38 fractions were isolated. Next, a part of the separated total fraction (Fr1-38) was taken out, and a developing solvent (chloroform-methanol-water ( 4:1:0.1)) unfolded (expanded distance: 8cm). After irradiating the TLC plate with UV (254 nm), sprayin...

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Abstract

To provide a Grifola frondosa extract which more effectively promotes the production of hyaluronic acid (hyaluronan) and a composition containing the same. A G. frondosa extract obtained by extracting dry G. frondosawith pure ethanol and a composition for promoting the production of hyaluronic acid (hyaluronan) which contains the same.

Description

technical field [0001] The present invention relates to a maitake extract and a composition containing it for promoting hyaluronic acid (hyaluronic acid) production. Background technique [0002] As ethanol extracts of maitake mushrooms, tyrosinase inhibitory effects, α-amylase inhibitory effects, emollient effects, and immunosuppressive effects have been known (Patent Documents 1 to 3). [0003] The present inventors proposed that the pure ethanol extract of the dried maitake fruiting body exhibits a cortex production and secretion promotion effect due to the promotion of cortex biosynthesis in human and hamster sebaceous glands, and a clinical improvement effect on skin morbidity in patients with xeroderma filed a patent application. That is, a patent application has been filed on the following content: the pure ethanol extract of dried maitake fruiting bodies improves the skin barrier function by promoting sebum production, and is effective not only for daily skin care b...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P17/00A61P17/02A61P17/06A61P17/16A61P19/02A61P27/02A61P37/08A61P43/00
CPCA61K36/07A61P17/00A61P17/02A61P17/06A61P17/16A61P19/02A61P27/02A61P37/08A61P43/00
Inventor 高桥雅夫伊东晃佐藤隆
Owner HEIMAT
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