Crystallizing method of 5'-guanosine-monophosphate disodium salt

A technology of disodium phosphate and guanosine, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of solid-liquid centrifugal separation, difficulty in clumping and sieving, and solubility reduction, etc., to achieve Prevent sieving difficulty, bright appearance, uniform particle effect

Active Publication Date: 2014-03-12
大连珍奥生物技术股份有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The ethanol solvent reduces the solubility of 5'-GMP and 2Na in the solution, resulting in the violent precipitation of 5'-GMP and 2Na. The precipitated product is a white powder or small crystal with high viscosity, and it is difficult to separate the solid and liquid by centrifugation.
The obtained product has problems such as uneven particles, strong hygroscopicity, easy clumping and difficulty in sieving, low purity and poor fluidity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Take 1000L of 5'-guanosine monophosphate aqueous solution with a concentration of 180g / L, adjust the pH to 7.5 with sodium hydroxide, then add 2000L of absolute ethanol and catalyst, and stir at 35°C to precipitate 5'-GMP and 2Na . The catalyst is a mixture of 5'-guanosine-phosphate disodium salt crystal 60-120 mesh sieve and sodium carbonate, 5'-guanosine-phosphate disodium salt crystal 60-120 mesh sieve and sodium carbonate The mass ratio is 1:2, and the amount of catalyst added is 1-2‰ of the mass of 5'-GMP. After the precipitate was centrifuged for solid-liquid separation, the resulting white crystals were washed with 85% ethanol and dried to obtain 193.0 kg of 5'-guanylic acid sodium salt crystals. The purity of the finished product checked with a liquid chromatograph is 99.4%, the moisture content is 17%, and the final crystallization yield is 90%.

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PUM

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Abstract

The invention discloses a crystallizing method of a 5'-guanosine-monophosphate disodium salt, which has the advantages of simple operation as well as large crystalline particle, uniform particle, attractive fineness and high purity of products. The method comprises the following steps of: adding sodium hydroxide to a 5'-GMP aqueous solution of which the concentration is 5-40 percent; regulating the pH value of the solution to 7-11 and then adding absolute alcohol which has a pH value of 7-11 and is 0.5-3 times of the solution in volume and a catalyst; stirring at 15-80 DEG C to separate out 5'-GMP, 2Na; and centrifuging, washing and drying to obtain 5'-GMP, 2Na crystals, wherein the catalyst is a mixture of a 60-120 mesh siftage of 5'-guanosine-monophosphate disodium salt crystals and sodium carbonate, the mass ratio of the 60-120 mesh siftage of 5'-guanosine-monophosphate disodium salt crystals to the sodium carbonate is 1:2, and the addition quantity of the catalyst is 0.1-0.2 percent of the mass of the 5'-GMP.

Description

Technical field: [0001] The invention relates to a crystallization purification method, in particular to a crystallization method of 5'-guanosine-phosphate disodium salt with simple operation, large product crystallization, good fluidity, uniform particles, beautiful color and high purity. Background technique: [0002] 5'-nucleotides (CMP, AMP, UMP, GMP, IMP) are products obtained by enzymatic degradation of RNA, and have been widely used in the fields of medicine, chemical industry, food, health care products and agriculture, such as the international infant milk powder industry The standard stipulates that 5'-nucleotides must be added, and the purity of 5'-nucleotides has always been a difficult problem in industrial production. [0003] At present, the known purification method of 5'-guanosine-phosphate disodium salt (5'-GMP, 2Na) is a conventional crystallization method, i.e. ethanol elution method. First use sodium hydroxide to adjust a certain concentration and tempe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/20C07H1/00C07H1/06
Inventor 陈玉松陈玉平孙明贤李威
Owner 大连珍奥生物技术股份有限公司
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