Process for extracting alpha-crocin from fructus gardeniae

A technology of crocin and gardenia, applied in the field of high color value α-crocin, can solve the problems of poor safety and unsuitability for industrial production, achieve low production cost, realize industrial production, and reduce workload

Inactive Publication Date: 2010-10-20
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on gardenia mainly focuses on the pharmacological effects of α-crocin, and there are few studies on the extraction technology of α-crocin. Chinese patent CN100537586C discloses "a kind of production of high-purity Purification method of yellow pigment", which adopts macroporous resin method and silica gel column chromatography to produce, but uses a large amount of toxic organic solvents such as petroleum ether, chloroform, methanol, etc., has poor safety, and is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Grind jasmine jasmine to 40 mesh, put 20kg into the extraction tank, add 30L No. 4 solvent, soak and heat to 40°C and stir for 1 hour, filter the degreasing liquid, add 30L of 50°C hot water to the degreasing raw material, adjust the pH to 4.8, add 200g of starch After enzymatic hydrolysis for 1 hour, quickly raise the temperature to 90°C and keep the temperature for half an hour, add water to 100L, adjust the pH to 7, heat and reflux for extraction for 3 hours, extract twice, filter while hot, combine the filtrate, add 7kg polyamide resin and stir for 0.5 hour, Filter to obtain 170L extract. Take HPD100 macroporous resin for column packing, pretreatment, then load the extract at a flow rate of 2BV / h, first elute with 4 times the column volume of deionized water, discard the eluent, and then successively use 6 times the column volume of 20% Elute with ethanol and 5 times column volume of 50% ethanol, detect by TLC, collect α-crocin eluate, recover ethanol by vacuum dist...

Embodiment 2

[0023] Crush jasmine jasmine to 50 mesh, put 20kg into the extraction tank, add 20L No. 4 solvent, soak and heat to 45°C and stir for 1.5 hours, filter the degreasing liquid, add 40L 50°C hot water to the degreasing raw material, adjust pH to 4.5, add 300g protease After 1 hour of enzymatic hydrolysis, quickly raise the temperature to 95°C and keep the temperature for half an hour, add water to 160L, adjust the pH to 7, heat and reflux for extraction for 2.5 hours, extract twice, filter while hot, combine the filtrates, add 7kg polyamide resin and stir for 0.5 hours, filter , to obtain 290L extract. Take HPD300 macroporous resin for column packing, pretreatment, then load the extract liquid at a flow rate of 1.5BV / h, first elute with 6 times the column volume of deionized water, discard the eluent, and then successively use 7 times the column volume Elute with 30% ethanol and 4 times column volume of 60% ethanol, detect by TLC, collect α-crocin eluate, recover ethanol by vacuu...

Embodiment 3

[0025] Grind the water gardenia to 50 mesh, put 20kg into the extraction tank, add 30L No.4 solvent, soak and heat to 45°C and stir for 1 hour, filter the degreasing liquid, add 36L of 50°C hot water to the degreasing raw material, adjust the pH to 4.8, and add 300g protease After 1 hour of enzymatic hydrolysis, quickly raise the temperature to 95°C and keep the temperature for half an hour, add water to 140L, adjust the pH to 7, heat and reflux for extraction for 3 hours, extract 3 times, filter while hot, combine the filtrate, add 7kg of polyamide resin and stir for 0.5 hours, filter , to obtain 380L extract. Take HPD100 macroporous resin for column packing, pretreatment, then load the extract at a flow rate of 1.5BV / h, first elute with 5 times column volume deionized water, discard the eluent, and then sequentially use 8 times column volume 20 % ethanol and 6 times of column volume 45% ethanol for elution, TLC detection, collect α-crocin eluate, recover ethanol by vacuum di...

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Abstract

The invention relates to a process for extracting alpha-crocin from fructus gardeniae, which comprises the following steps of degreasing, aqueous extraction, tannin removal, resin adsorption, recrystallization and the like, and particularly comprises a method of degreasing by a No.4 solvent, tannin removal by polyamide resin and the recrystallization of hot water and propylene glycol. The production process has the characteristics of simplicity, convenience and low cost.

Description

Technical field: [0001] The invention relates to a process for extracting α-crocin from gardenia, in particular to producing high-value α-crocin by using the methods of degreasing with No. 4 solvent, removing tannin with polyamide resin, and separating and enriching macroporous resin. Background technique: [0002] α-crocin (crocin), molecular formula: C 44 h 64 o 24 ; Molecular weight 976.96; Melting point 186°C. [0003] Physical and chemical properties: Brown-red needle-like crystal, easily soluble in hot water, soluble in acetic acid and propylene glycol, slightly soluble in absolute ethanol, ether and other organic solvents. It is a small number of water-soluble carotenoids. [0004] Gardenia is the dry and mature fruit of Gardenia jasminoides of Rubiaceae. It is divided into gardenia and water gardenia, mainly distributed in Zhejiang, Jiangxi, Fujian, Anhui, Hunan and other places. It has the effects of purging fire and relieving restlessness, clearing heat and da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/44
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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