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Tissue culture method of golden-leaf yucca

A technique for tissue culture and yucca japonica, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of the small number of yucca introductions, limited supply of seedlings, root tiller propagation, and slow propagation of ramets and other problems, to achieve the effect of improving the propagation speed, the uniformity of the seedlings, and improving the stability

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced species of Yucca japonica is relatively small, and its root tiller propagation and branch propagation are slow, so the supply of seedlings is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water, use sterile filter paper to blot the water on the surface of the buds, cut the buds into 0.5cm-long segments with buds, and inoculate the segments with MS+6-BA0.5mg / L+NAA0 .1 mg / L bud induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] Two weeks after the segments were inoculated on the bud induction medium, the buds began to swell, and yellow-green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for another month, the buds could grow to 1 cm. The adventitious buds were cut off and transferred to Into the adventitious bud proliferation medium compr...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and then soak them in mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water, use sterile filter paper to dry the water on the surface of the buds, cut the buds into 1cm-long segments with buds, and inoculate the segments with MS+6-BA3.0mg / L+NAA0. 3mg / L bud induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the bud induction medium for 3 weeks, the buds began to expand, and yellow-green protrusions appeared. After 4 weeks, bud meristems could be seen. After one and a half months of culture, the buds could grow to 3cm. Cut off the adventitious buds and transfer them to Into the adventitious bud proliferation medium compri...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young shoots, remove the wrapped leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and mercury with a volume concentration of 2‰ for 50 minutes, and then Rinse 6 times with sterile water, use sterile filter paper to dry the water on the surface of the buds, cut the buds into 2cm-long segments with buds, and inoculate the segments with MS+6-BA3.0mg / L+NAA0. 3mg / L bud induction medium;

[0054](2) Differentiation and proliferation of buds

[0055] After the segments were inoculated on the bud induction medium for 4 weeks, the buds began to expand, and yellow-green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the buds could grow to 2cm. Cut off the adventitious buds and transfer them to Into the adventitious bud proliferation medium comprising MS+6-BA2.0mg / L+NAA...

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PUM

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Abstract

The invention relates to a tissue culture method of golden-leaf yucca, comprising the steps of: acquisition of bacteria-free material, differentiation and proliferation of bud, sound seeding of adventitious bud, rooting culture, hardening and transplantation and the like. Compared with the prior art, the tissue culture method greatly enhances reproduction speed of golden-leaf yucca and uniformity of seedlings, raises stability of characters thereof, and can realize batch production of seedlings in factory.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Yucca aureus. Background technique [0002] Golden leaf yucca is a plant of the genus Yucca in the family Liliaceae, a small evergreen shrub, with wrinkles on the leaves, dark green and covered with a small amount of white powder, with yellow stripes, and a few filaments on the old leaves. It blooms in summer and autumn, and has strong drought resistance. Yucca is an ornamental plant with beautiful flowers and leaves. It has strong resistance and absorption capacity to harmful gases such as sulfur dioxide, hydrogen fluoride, chlorine, ammonia, etc. It is also a good flower mirror and garden material. However, as a new variety introduced from abroad, the number of introduced species of Yucca japonica is relatively small, and its root tiller propagation and division propagation are slow, so the supply of seedlings is limited. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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